Abstract
This survey is aimed at the assessment of the possible effect of different plant growth regulators (PGRs) on the micropropagation of commercial sweet cherry rootstocks Gisela 12 (Prunus cerasus × Prunus canescens), and Maxma 14 (Prunus avium × Prunus mahaleb). To study Gisela 12 and Maxma 14 micropropagation were assessed utilizing lateral buds. The treatments used for the establishment and shoots proliferation included MS medium fortified by benzyl amino purine (BAP) (0.0, 0.5, 1.0, 1.5 and 2.0 mg L−1) and indol-3-butyric acid (IBA) (0, 0.25, 0.50 and 1.0 mg L−1). For rooting, IBA was used at four levels (0.0, 0.25, 0.50, and 1.0 mg L−1) in combination with 0.0, 0.25, and 0.50 mg L−1 naphthaleneacetic acid (NAA) in full and half-strength MS media. Maximum shoot multiplication for Gisela 12 (6.2 shoots/explants) and Maxma 14 (4.4 shoots/explants) were observed on MS medium reinforced with 2.0 mg L−1 BAP with 0.25 mg L−1 IBA. The best rooting response of Gisela 12 (100%) and Maxma 14 (88.88%) was also achieved on half-strength MS including 1.0 mg L−1 IBA plus 0.25 NAA and 0.5 IBA plus 0.25 NAA, respectively. The ISSR banding profile showed the genetic stability of propagated plants along with the mother plant. This is the first study evaluating the use of molecular markers for genetic uniformity test, which can be applied for large-scale propagation.
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References
Aghaye RNM, Yadollahi A, Moeini A, Sepahvand S (2013) In vitro culture of Gisela 6 semi-dwarf rootstock. J Bio Env Sci 7:57–64
Aremu AO, Bairu MW, Dolezal K, Finnie JF, Van Staden J (2012) Topolins: a panacea to plant tissue culture challenges? Plant Cell Tiss Org 108:1–16. https://doi.org/10.1007/s11240-011-0007-7
Arnon DI (1949) Copper enzymes in isolated chloroplasts. polyphenoloxidase in Beta vulgaris. Plant Physiol 24:1–15. https://doi.org/10.1104/pp.24.1.1
Bartrina I, Otto E, Strnad M, Werner T, Schmulling T (2011) Cytokinin regulates the activity of reproductive meristems, flower organ size, ovule formation, and thus seed yield in Arabidopsis thaliana. Plant Cell 23:69–80. https://doi.org/10.1105/tpc.110.079079
Bhatia R, Singh KP, Sharma TR, Jhang T (2011) Evaluation of the genetic fidelity of in vitro propagated gerbera (Gerbera jamesonii Bolus) using DNA based markers. Plant Cell Tiss Org Cult 104:131–135. https://doi.org/10.1007/s11240-010-9806-5
Bishopp A, Benkova E, Helariutta Y (2011) Sending mixed messages: auxin-cytokinin crosstalk in roots. Curr Plant Biol. https://doi.org/10.1016/j.pbi.2010.08.014
Bosnjak AM, Keresa S, Jercic IH, Baric M (2012) The effect of cytokinin type and explant orientation on axillary shoot proliferation and in vitro rooting of ‘Gisela 5’ cherry rootstock. J Food Agric Environ 10(3):616–620. https://doi.org/10.22059/ijhs.2018.248845.1370
Chauhan HK, Bisht AK, Singh L (2015) Effects of phytohormones BA (6-benzylaminopurine) and IBA (Indole-3-butyric acid) on shoot and root multiplication in Allium hookeri Thw. Int J Environ Sci Technol 10:87–93
Cui Y, Deng Y, Zheng K, Hu X, Zhu M, Deng X, Xi R (2019) An efficient micropropagation protocol for an endangered ornamental tree species (Magnolia sirindhorniae Noot. & Chalermglin) and assessment of genetic uniformity through DNA markers. Sci Rep 9:9634. https://doi.org/10.1038/s41598-019-46050-w
Das Bhowmik SS, Basu A, Sahoo L (2016) Direct shoot organogenesis from rhizomes of medicinal zingiber Alpinia calcarata Rosc. and evaluation of genetic stability by RAPD and ISSR markers. J Crop Sci Biotechnol 19:157–165. https://doi.org/10.1007/s12892-015-0119-4
Davies PJ (2010) The plant hormones: their nature, occurrence, and functions. Plant Hormones. https://doi.org/10.1007/978-1-4020-2686-7_1
Dimitrova MA, Yordanova ZP, Kapchina-Toteva VM (2012) Influence of 6-benzylaminopurine and indole-3-butyric acid on in vitro propagation and secondary metabolites accumulation in Lamium album from Lozen Mountain. Inter Soc Hort Sci 955:315–320. https://doi.org/10.17660/ActaHortic.2012.955.47
Doric M, Magazin N, Milic B, Keserovic Z (2015) Enhancing feathering of one-year-old Gala and Jonagold apple trees through application of 6-benzylaminopurine and gibberellins. Bulg J Agric Sci 21(3):631–637
Drkenda P, Spahic A, Spahic A, Begic-Akagic A (2012) Testing of ‘Gisela 5’ and ‘Santa Lucia 64’ cherry rootstocks in Bosnia and Herzegovina. Acta Agric Slov 99(2):129–136. https://doi.org/10.2478/v10014-012-0012-5
Fallahpour M, Miri SM, Bouzari N (2015) In vitro propagation of ‘Gisela 5’rootstock as affected by mineral composition of media and plant growth regulators. J Hort Res 23(1):57–64. https://doi.org/10.2478/johr-2015-0008
Fang JY, Hsu YR (2012) Molecular identification and antibiotic control of endophytic bacterial contaminants from micropropagated Aglaonema cultures. Plant Cell Tiss Org Cul 110:53–62. https://doi.org/10.1007/s11240-012-0129-6
Frugier F, Kosuta S, Murray JD, Crespi M, Szczyglowski K (2008) Cytokinin: secret agent of symbiosis. Trends Plant Sci 13:115–120. https://doi.org/10.1016/j.tplants.2008.01.003
Gao H, Li W, Yang J (2003) Effect of 6-benzyladenine and casein hydrolysate on micropropagation of Amorpha fruticose. Biol Plant 47:145–148. https://doi.org/10.1023/A:1027361721860
Goto S, Thakur RC, Ishii K (1998) Determination of genetic stability in long-term micropropagated shoots of Pinus thunbergii Parl using RAPD markers. Plant Cell Rep 18:193–197. https://doi.org/10.1007/s002990050555
Holtz B, Ferguson L, Allen GE (1995) Pistachio production, rootstocks production and budding. Cooperative extension University of California Oakland, pp 54–56
Hosseinpour B, Bouzari N, Didar Z, Masoumian M et al (2015) High frequency in vitro propagation of M × M60, a cherry rootstock: the effects of culture media and growth regulators. Iran J Genet Plant Breed 4(2):28–36
Isah T (2020) Nodal segment explant type and preconditioning influence in vitro shoot morphogenesis in Ginkgo biloba L. Plant Physiol Rep 25:74–86. https://doi.org/10.1007/s40502-019-00475-7
Kumar S, Singh H, Pandey V, Singh BD (2016) In vitro multiplication of pointed gourd (Trichosanthes dioica) through nodal explant culture, and testing the genetic fidelity of micropropagated plants using RAPD markers. Indian J Biotech 15:581–588. https://doi.org/10.3329/bjsir.v47i2.11457
Lazo-Javalera MF, Troncoso-Rojas R, Tiznado-Hernandez ME, Martinez-Tellez MA, Vargas-Arispuro I, Islas-Osuna MA, Rivera-Dominguez M (2016) Surface disinfection procedure and in vitro regeneration of grapevine (Vitis vinifera L.) axillary buds. Springer Plus 5:453. https://doi.org/10.1186/s40064-016-2081-0
Liang Ch, Wu R, Han Y, Wan T, Cai Y (2019) Optimizing suitable antibiotics for bacterium control in micropropagation of cherry rootstock using a modified leaf disk diffusion method and e test. Plants 8(3):66. https://doi.org/10.3390/plants8030066
Liu X, Li Y, Zhong S (2017) Interplay between light and plant hormones in the control of Arabidopsis seedling chlorophyll biosynthesis. Front Plant Sci 8:1433. https://doi.org/10.3389/fpls.2017.01433
Long LE (2007) Four simple steps to pruning cherries on ‘Gisela’ and other productive rootstocks. Oregon State University, Corvallis
Maclachlan S, Zalik S (1963) Plastid structure, chlorophyll concentration, and free amino acid composition of a chlorophyll mutant of barley. Can J Bot 41(7):1053–1062
Mahdavian M, Bouzari N, Abdollahi H (2011) Effects of media and plant growth regulators on micropropagation of a dwarfing cherry rootstock (PHL-A). Biharean Biologist 5:60–90
Majda M, Robert S (2018) The role of auxin in cell wall expansion. Int J Mol Sci 19(4):951. https://doi.org/10.3390/ijms19040951
Mansseri-Lamrioui A, Louerguioui A, Abousalim A (2009) Effect of the medium culture on the micro cutting of material resulting from adult cuttings of wild cherry trees (Prunus avium L.) and in vitro germination. Eur J Sci Res 25:345–352
Mbah EI, Wakil SM (2012) Elimination of bacteria from in vitro yam tissue cultures using antibiotics. J Plant Pathol 94(1):53–58
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15(3):473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8(19):4321–4325. https://doi.org/10.1093/nar/8.19.4321
Parsaeimehr A, Sargsyan E, Javidnia K (2010) A comparative study of the antibacterial, antifungal and antioxidant activity and total content of phenolic compounds of cell cultures and wild plants of three endemic species of ephedra. Molecules 15(3):1668–1678. https://doi.org/10.3390/molecules15031668
Pop TI, Pamfil D, Bellini C (2011) Auxin control in the formation of adventitious roots. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 39(1):307–316. https://doi.org/10.15835/nbha3916101
Rathore JS et al. (2004) Micropropagation of woody plants. In: Srivastava PS, Narula A, Srivastava S (eds) Plant biotechnology and molecular markers. pp 195–205. https://doi.org/10.1007/1-4020-3213-7-13
Saha S, Adhikari S, Dey T, Ghosh P (2015) RAPD and ISSR based evaluation of genetic stability of micropropagated plantlets of Morus alba L. variety S-1. Meta Gene 7:7–15
Sansavini S, Lugli S (2009) New rootstocks: for intensive sweet cherry plantations. Int Soc Hortic Sci 1020:411–434
Saponari M, Bottalico G, Savino V (1999) In vitro propagation of Prunus mahaleb and its sanitation from prune dwarf virus. Hort Sci 13:56–60
Sarropoulou V, Dimassi-Theriou K, Therios I (2013) Effects of exogenous l-arginine on in vitro rooting, chlorophyll, carbohydrate, and proline concentrations in the sweet cherry rootstock M× M 14 (Prunus avium L.× Prunus mahaleb L.). Plant Biotech Rep 7:457–465. https://doi.org/10.1007/s11816-013-0284-1
Sharma V, Thakur M, Kumar A (2017) An efficient method for in vitro propagation of Gisela 5 (Prunus cerasus X Prunus canescens)—Clonal Cherry Rootstock. Int J Curr Microbiol Appl Sci 6:2617–2624. https://doi.org/10.20546/ijcmas.2017.608.311
Singh S, Sundouri A, Sharma M, Srivastava K, Dar H (2010) Proliferation and rooting efficiency studies in sour cherry (Prunus cerasus) using in vitro techniques. J Hort Sci 5:48–52
Tarrahi R, Rezanejad F (2013) Callogenesis and production of anthocyanin and chlorophyll in callus cultures of vegetative and floral explants in Rosa gallica and Rosa hybrida (Rosaceae). Turk J Bot 37:1145–1154. https://doi.org/10.3906/bot-1205-42
Teixeira da Silva JA, Dobranszki J, Ross S (2013) Phloroglucinol in plant tissue culture. Vitro Cell Dev Bio Plant 49:1–16. https://doi.org/10.1007/s11627-013-9491-2
Tognetti VB, Mühlenbock P, Van Breusegem F (2012) Stress homeostasis–the redox and auxin perspective. Plant Cell Env 35:321–333. https://doi.org/10.1111/j.1365-3040.2011.02324.x
Trevisan S, Pizzeghello D, Ruperti B, Francioso O, Sassi A, Palme K, Quaggiotti S, Nardi S (2010) Humic substances induce lateral root formation and expression of the early auxin-responsive IAA19 gene and DR5 synthetic element in Arabidopsis. Plant Biol 12(4):604–614. https://doi.org/10.1111/j.1438-8677.2009.00248.x
Usenik V, Fajt N, Mikulic-Petkovsek M, Slatnar A, Stampar F, Veberic R (2010) Sweet cherry pomological and biochemical characteristics influenced by rootstock. J Agric Food Chem 58:4928–4933. https://doi.org/10.1021/jf903755b
Vanstraelen M, Benkova E (2012) Hormonal interactions in the regulation of plant development. Annu Rev Cell Dev Biol 28:463–487. https://doi.org/10.1146/annurev-cellbio-101011-155741
Vidoz ML, Loreti E, Mensuali A, Alpi A, Perata P (2010) Hormonal interplay during adventitious root formation in flooded tomato plants. Plant J 63(4):551–562. https://doi.org/10.1111/j.1365-313X.2010.04262.x
Wang H, Nair MG, Strasburg GM et al (1999) Antioxidantand anti-inflammatory activities of anthocyanins and their aglycon, cyanidin, from tart cherries. J Nat Prod 62(2):294–296. https://doi.org/10.1021/np980501m
Webster AD (1996) The taxonomic classification of sweet and sour cherries and a brief history of their cultivation. In: Webster AD, Looney NE (eds) Cherries: crop physiology, production and uses. CAB International, Wallingford, pp 3–24
Zaerr JB, Mapes MO (1982) Action of growth regulators. In: Bonga JM, Durzan DJ (eds) Tissue culture in forestry. Martinus Nijhoff/Dr. W. Junk Publishers, The Hague, pp 231–255
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Author contributions AMJ and SPP conducted the experiments, sampling, and data analysis, as well as writing the primary draft of the manuscript. BS and AHHM were the supervisors of the research and contributed to writing the final version of the manuscript.
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Jafarlou, A.M., Pirivatlo, S.P., Salehi, B. et al. Establishing an efficient in vitro propagation system for sweet cherry rootstocks Gisela 12 and Maxma 14 and assessment of genetic homogeneity by ISSR markers. J. Crop Sci. Biotechnol. 24, 449–460 (2021). https://doi.org/10.1007/s12892-021-00093-6
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DOI: https://doi.org/10.1007/s12892-021-00093-6