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Elucidate Genetic Diversity and Population Structure of Bread Wheat (Triticum Aestivum L.) Cultivars Using IRAP and REMAP Markers

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Abstract

Analysis of genetic diversity and population structure in bread wheat is an essential step in their conservation, utilization, and breeding. Retrotransposons are ubiquitous and abundant a throughout the plant genomes, therefore extensively used as ideal molecular markers for genetic variability, DNA fingerprinting and genetic mapping studies in plant species. In the current research, we used two retrotransposon-based marker systems, inter-retrotransposon amplified polymorphisms (IRAPs), and the retrotransposon-microsatellite amplified polymorphisms (REMAPs) markers to evaluate the genetic diversity and survey activity of long terminal repeat retrotransposon (LTR-retrotransposon) elements in a collection of 49 bread wheat (Triticum aestivum L.) cultivars that mainly bred in Iran. In general, 90 and 126 loci were amplified using 9 IRAP and 20 REMAP primers, respectively. Both techniques produced a satisfactory number of bands for cultivar analysis; however, the technique IRAP, particularly single primer Nikita generated a large number of bands, indicating the wide activity of Nikita family under various environmental conditions of bread wheat. The percentage of polymorphic loci (PPL) in the studied collection for IRAP and REMAP markers was 81.78 and 86.40%, respectively. A model-based Bayesian method, Principal coordinate analysis (PCoA) and cluster analysis using Minimum Evolution (ME) algorithm hinted of the existence of two groups. This grouping was in agreement with the growing season and conformed by the high within-group bootstrap value. These results demonstrated that these markers developed using transpositionally active retrotransposons (RTNs) are efficient and reliable markers in determining level of genetic diversity and population structure in bread wheat in breeding programs.

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Authors and Affiliations

  1. Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Tabriz University, Tabriz, Iran

    Hossein Abbasi Holasou

  2. Department of Genetic, Faculty of Science, Islamic Azad University Tabriz Branch, Tabriz, Iran

    Frough Rahmati

  3. Department of Biology and Institute of Biotechnology, Faculty of Sciences, Urmia University, Urmia, Iran

    Fatemeh Rahmani

  4. Divison of Biochemistry, Department of Basic Science, Faculty of Bacis Science, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

    Mahdi Imani

  5. Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Urmia University, Urmia, Iran

    Zoleikha Talebzadeh

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  1. Hossein Abbasi Holasou
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  2. Frough Rahmati
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Correspondence to Hossein Abbasi Holasou.

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Abbasi Holasou, H., Rahmati, F., Rahmani, F. et al. Elucidate Genetic Diversity and Population Structure of Bread Wheat (Triticum Aestivum L.) Cultivars Using IRAP and REMAP Markers. J. Crop Sci. Biotechnol. 22, 139–151 (2019). https://doi.org/10.1007/s12892-018-0211-0

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