Abstract
This study was executed to evaluate the phenolic content, DPPH, ABTS radical scavenging rate, nitrite scavenging rate, and anti-inflammatory activity from in vitro cultured adventitious root extract of different Platycodon grandiflorum(PG) variety. Total polyphenol and flavonoid content showed the highest amount in adventitious root extract of tetraploid PG, and followed by green petal, double petal and diploid PG extract. The DPPH radical scavenging activity showed that the increase was proportional to the concentration, especially, the scavenging activity at a concentration of 10 mg mL-1 was higher than 80%. The ABTS radical scavenging activity was not significantly different among the PG varieties at the concentration of 5 mg mL-1 or more, and generally showed high scavenging activity. The nitrite scavenging activity was affected by pH, at pH of 1.2, the scavenging effect of all of the extracts tested observed higher than that of the other two pH ranges. The cytotoxicity of PG extract at various concentrations, the survival rate of RAW 264.7 cell was more than 90% at a concentration of 200 μg mL-1 or less, and it was confirmed that the macrophage cell showed little toxicity. After PG extract pretreatment at 50, 100, and 200 μg mL-1, LPS-treated experimental group significantly inhibited NO production in a dose-dependent manner. The dose-response trends followed quadratic regressions in all PG varieties. The PG extracts showed a considerable range of influence on cytokine secretion. The effect of adventitious root extracts of PG against inflammatory mediators TNF-α, IL-6 and IL-1β production showed the most significant anti-inflammatory activity.
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Ajuebor MN, Virag L, Flower RJ, Perretti M, Szabo C. 1998. Role of inducible nitric oxide synthase in the regulation of neutrophil migration in zymosan-induced inflammation. Immunology. 95: 625–630
Balkwill F, Charles KA, Mantovani A. 2005. Smoldering and polarized inflammation in the initiation and promotion of malignant disease. Cancer Cell. 7(3): 211–217
Boo HO, Shin JH, Shin JS, Choung ES, Bang MA, Choi KM, Song WS. 2012. Assessment on Antioxidant Potential and Enzyme Activity of Some Economic Resource Plants. Korean J. Plant Res. 25(3): 349–356
Boora F, Chirisa E, Mukanganyama S. 2014. Evaluation of Nitrite Radical Scavenging Properties of Selected Zimbabwean Plant Extracts and Their Phytoconstituents. J. Food Process. 2014
Brand-Williams W, Cuvelier ME, Berset C. 1995. Use of a free radical method to evaluate antioxidant activity. Food Sci. Tech. 28: 25–30
Chen LG, Yang LL, Wang CC. 2008. Anti-inflammatory activity of mangostins from Garcinia mangostana. Food and Chemical Toxicology. 46(2): 688–693
Choi DB, Cho KA, Na MS, Choi HS, Kim YO, Lim DH, Cho SJ, Cho H. 2008. Effect of bamboo oil on antioxidative activity and nitrite scavenging activity. J. Ind. Eng. Chem. 14: 765–770
Choi JH, Hwang YP, Lee HS, Jeong HG. 2009. Inhibitory effect of Platycodi Radix on ovalbumin-induced airway inflammation in a murine model of asthma. Food Chem Toxicol. 47: 1272–1279
Feldmann M, Brennan FM, Maini RN. 1996. Role of cytokines in rheumatoid arthritis. Annual Review of Immunology. 14: 397–440
Fu XJ, Liu HB, Wang P, Guan HS. 2009. A study on the antioxidant activity and tissues selective inhibition of lipid peroxidation by saponins from the roots of Platycodon grandiflorum. Am. J. Chin. Med. 37(5): 967–975
Gao SL, Zhu DN, Cai ZH, Xu DR. 1996. Autotetraploid plants from colchicine-treated bud culture of Salvia miltior-rhiza Bge. Plant Cell, Tissue, and Organ Cult. 47(1): 73–77
Gülcin I, Huyut Z, Elmastas M, Aboul-Enein A. 2010. Radical scavenging and antioxidant activity of tannic acid. Arabian Journal of Chemistry. 3: 43–53
Guo LY, Hung TM, Bae kH, Shin EM, Zhou HY, Hong YN, Kang SS, Kim HP, Kim YS. 2008. Anti-inflammatory effects of schisandrin isolated from the fruit of Schisandra chinensis Baill. European Journal of Pharmacology. 591(1-3): 293–299
Hong TG, Lee YR, Yim MH, Hyun CN. 2004. Physiological functionality and nitrite scavenging ability of fermentation extracts from pine needles. Korean J Food Preserv. 11: 94–99
Ialenti A, Moncada S, Di Rosa M. 1993. Modulation of adjuvant arthritis by endogenous nitric oxide. Br J Pharmacol. 110: 701–706
Jang JR, Hwang SY, Lim SY. 2011. Inhibitory effect of extracts of Platycodon grandiflorum (the Ballon Flower) on oxidation and nitric oxide production. Korean J Food Preserv. 18: 65–71
Jeong HM, Han EH, Jin YH, Hwang YP, Kim HG, Park BH, Kim JY, Chung YC, Lee KY, Jeong HG. 2010. Saponins from the roots of Platycodon grandiflorum stimulate osteoblast differentiation via p38 MAPK-and ERK-dependent RUNX2 activation. Food Chem Toxicol. 48: 3362–3368
Joseph JA, Shukitt-Hale B, Lau FC. 2007. Fruit polyphenols and their effects on neuronal signaling and behavior in senescence. In Annals of the New York Academy of Sciences. Vol. 1100. pp 470–485
Ju JH, Kim JS, Kang SS, Son KH, Chang HW, Kim HP. 2010. Anti-inflammatory and anti-arthritic activity of total flavonoids of the roots of Sophora flavescens. Journal of Ethnopharmacology. 127(3): 589–595
Karin M, Ben-Neriah Y. 2000. Phosphorylation meets ubiquitination: The control of NF-B activity. Annual Review Journal of Immunology. 18: 621–663
Kato H, Lee IE, Van Chuyen N, and Kim F. Hayase. 1987. Inhibition of nitrosamine formation by nondialyzable melanoidins. Agr. Biol. Chem. 51: 1333–1338
Kim ID, Dhungana SK, Kim HR, Shin DH. 2017. Quality Characteristics and Antioxidant Potential of Seeds of Native Korean Persimmon Genotypes. Korean J. Plant Res. 30(6): 670–678
Kim CH, Jung BY, Jung SK, Lee CH, Lee HS, Kim BH, Kim SK. 2010. Evaluation of antioxidant activity of Platycodon grandiflorum. J. Environ. Toxicol. 25(1): 85–94
Leong LP, SHhui G. 2002. An investigation of antioxidant capacity of fruits in Singapore markets. Food Chem. 76: 69–75
Marja PK, Anu IH, Heikki JV, Jussi-Pekka R, Kalevi P, Tytti SK, Marina H. 1999. Antioxidant activity of plant extracts containing phenolic compounds. J. Agri. Food Chem. 47: 3954–3962
Nyakudya E, Jeong JH, Lee NK, Jeong YS. 2014. Platycosides from the roots of Platycodon grandiflorum and their health benefits. Prev Nutr Food Sci. 19: 59–68
Pokharel YR, Liu QH, Oh JW, WOO R, Kang KW. 2007. 4-Hydroxykobusin inhibits the induction of nitric oxide synthase by inhibiting NF-kB and AP-1 Activation. Biological and Pharmaceutical Bulletin. 30: 1097–1101
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Evans CR. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Bio. Med. 26(9-10): 1231–1237
Rice-Evans C, Miller NJ, Bolwell GP, Bramley PM, Pridham JB. 1995. The relative antioxidants activities of plant-derived polyphenolic flavonoids. Free Radic. Res. 22: 375–383
Ryu CS, Kim CH, Lee SY, Lee KS, Choung KJ, Song GY, Kim BH, Ryu SY, Lee HS, Kim SK. 2012. Evaluation of the total oxidant scavenging capacity of saponins isolated from Platycodon grandiflorum. Food Chem. 132(1): 333–337
Sanchez-Moreno C, Larrauri JA, Saura-Calixto F. 1999. Free radical scavenging capacity and inhibition of lipid oxidation of wines, grape juices and related polyphenolic constituents. Food Res. Int. 32: 407–412
Sautebin L, Ialenti A, Ianaro A, Di Rosa M. 1998. Relationship between nitric oxide and prostaglandins in carrageenin pleurisy. Biochem Pharmacol 55: 1113–1117
Shahidi F, Wanasundara PKJPD. 1992. Phenolic antioxidants: criteria review. Food Science and Nutrition 32: 67–103
Shin JH, Lee JY, Ju JC, Lee SJ, H.S. Cho HS, Sung NJ. 2005. Chemical properties and nitrite scavenging ability of citron (Citrus junos). J. Korean Soc. Food Sci. Nutr. 34: 496–502
Singleton V, Rossi JA. 1965. Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents. Am. J. Enology Vitic. 16: 144–158
Sugihara N, T. Arakawa T, Ohnishi M, Furuno K. 1999. Anti and pro-oxidative effects of flavonoids on metal induced lipid hydroperoxide-dependent lipid peroxidation in cultured hepatocytes located with "-linolenic acid. Free Radic. Biol. Med. 27: 1313–1323
Sung N, Lee S, Shin J, Lee I, Chung Y. 1996. Effects of Platycodon grandiflorum extract on blood glucose and lipid composition in alloxan induced hyperglycemic rats. J Korean Soc Food Sci Nutr. 25: 986–992
Takagi K, Lee EB. 1972. Pharmacological studies on Platycodon grandiflorum A. DC. 3. Activities of crude platycodin on respiratory and circulatory systems and its other pharmacological activities. Yakugaku Zasshi. 92: 969–973
Thao, N.T.P., K. Ureshino, I. Miyajima, Y. Ozaki and H. Okubo. 2003. Induction of tetraploids in ornamental Alocasia through colchicine and oryzalin treatments. Plant Cell, Tissue and Organ Culture. 72: 19–25
Velioglu YS, Mazza G, Gao L, Oomah BD. 1998. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J. Agric. Food. Chem. 46: 4113–4117
Willeaume V, Kruys V, Mijatovic T, Huez G. 1996. Tumor necrosis factor-alpha production induced by viruses and by lipopolysaccharides in macrophages: Similarities and differences. Journal of Inflammation. 46: 1–12
Yokozawa T, Chen CP, Dong E, Tanaka T, Nonaka GI, Nishioka I. 1998. Study on the inhibitory effect of tannins and flavonoids against the 1,1-diphenyl-2-picrylhydrazyl radical. Biochem. Phamacol. 56: 213–222
Zhao F, Wang L, Liu K. 2009. In vitro anti-inflammatory effects of arctigenin, a lignan from Arctium lappa L., through inhibition on iNOS pathway. Journal of Ethnopharacology. 122: 457–462
Zhishen J, Mengcheng T, Jianming W. 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 64: 555–559
Zhou K, Yu L. 2006. Total phenolic contents and antioxidant properties of commonly consumed vegetables grown in Colorado. LWT. 39: 1155–1162
Zhu N, Wang M, Wei GJ, Lin JK, Yang CS, Ho CT. 2001. Identification of reaction products of (-)-epigallocatechin, (-)-epigallocatechin gallate and pyrogallol with 2,2-diphenyl-1-picrylhydrazyl radical. Food Chem. 73: 345–349
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Boo, H.O., Park, J.H., Kim, H.H. et al. Evaluation of Physiological Functionalities and Anti-inflammatory Activity on in vitro Cultured Adventitious Root of Platycodon grandiflorum. J. Crop Sci. Biotechnol. 21, 183–191 (2018). https://doi.org/10.1007/s12892-018-0101-0
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DOI: https://doi.org/10.1007/s12892-018-0101-0