Journal of Crop Science and Biotechnology

, Volume 21, Issue 1, pp 43–55 | Cite as

Comparative Germplasm Characterization of Maize (Zea mays L.) in Rajouri Region of Pir Panjal Himalaya J & K (India), based on Morphological and ISSR Markers

  • Tanvir H. Dar
  • Rubiada Shakeel
  • Shusheel Verma
Research Article


The present study provides an assessment of genetic variability and relationship within and between different cultivars of maize grown across the Rajouri region of the Pir Panjal Himalaya, utilizing morphological and ISSR markers. Morphological descriptors showed significant diversity among cultivars; on morphological cluster analysis, the cultivars 9 and 7 were more related, while cultivars 10 and 3 were distinct. Fifty accessions (5 from each cultivar) were characterized using a DNA-based molecular marker, ISSR. In all, 108 amplification products were generated with 17 ISSR primers, 6.35 fragments with an average \per primer. Out of these, 83 were found to be polymorphic with an overall percentage polymorphism of 75.2%. Total genetic diversity (Ht) and the mean genetic diversity estimated within populations (HS) was 0.2613 and 0.0803, respectively. Gene flow (Nm) and Coefficient of gene differentiation (Gst) among the cultivars was 0.2220 and 0.6926, respectively. Nei’s genetic diversity index (H) and (I) were the lowest for cultivar 10 (H = 0.037; I = 0.056) and the highest for cultivar 8 (H = 0.121; I = 0.176). Analysis of molecular variance revealed 35% within group and 65% among group genetic diversity. Based on cluster analysis, cultivar 10 appeared distinct from rest of the cultivars. The high genetic diversity detected in the present study can be utilized in maize breeding programs, wherein the elite genotypes could be crossed with the existing cultivars to form novel gene and trait combinations.

Key words

Genetic diversity morphological descriptors ISSR markers maize germplasm 


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Copyright information

© Korean Society of Crop Science and Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Tanvir H. Dar
    • 1
  • Rubiada Shakeel
    • 1
  • Shusheel Verma
    • 1
  1. 1.School of Biosciences & BiotechnologyBaba Ghulam Shah Badshah UniversityRajouriIndia

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