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Journal of Crop Science and Biotechnology

, Volume 21, Issue 1, pp 89–94 | Cite as

Efficient Micropropagation Protocol for Jatropha Curcas Using Liquid Culture Medium

  • Aneesha Singh
Research Article
  • 99 Downloads

Abstract

Although several studies have been made on the micropropagation of Jatropha curcas using agar base mediums, none of them have been by using liquid medium systems. The effects of explant type and temporary immersion system (test tube, jar with filter paper boat, and growtek bioreactor) on the micropropagation of J. curcas were studied. The explant type influenced shoot quality, multiplication coefficient (MC), and rooting. Leaf explant produced more and longer shoots than nodal explant. Use of filter paper (FB) boat prevented hyperhydricity and allowed proliferation of nodal explants cultured in liquid MS (Murashige and Skoog) medium supplemented 6-benzylaminopurine (BAP) and Kinetin (KN). The best shoot bud induction (92.1±3.1%) was achieved in liquid MS medium supplemented with 2.0 mg/L KN. Leaf regeneration efficiency was compared in growtek bioreactor and in jar containing liquid MS medium supplemented with 0.5 mg/L Thidiazuron (TDZ). The best shoot bud regeneration (78.7±2.1%) was obtained in growtek bioreactor. Shoot buds achieved from nodal segment and leaf were subcultured on filter paper boats in jar and bioreactor containing liquid MS medium supplemented with BAP, Indole butyric acid (IBA), Indole-3-acetic acid (IAA), and KN. Best shoot proliferation and elongation was obtained in filter paper boats containing liquid MS medium supplemented with 1.5 mg/L BAP, 0.5 mg/L IAA, and 0.2 mg/L KN. The number of multiple shoot buds was higher in leaf explants as compared to nodal explants and the highest number of multiple shoot buds was recorded from leaf explants. Up to 76.4% rooting efficiency was obtained when the shoots were ex vitro rooted. The generated plants well established in the nursery and grew normally in outdoor conditions. The protocol has good potential for application in large-scale propagation of J. curcas using liquid medium.

Key words

Cotton filter paper boat bioreactor liquid medium jatropha 

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References

  1. Aitken-Christie J. 1991. Automation In: PC Debergh, RH Zimmerman, eds., Micropropagation. Kluwer, Dordrecht, pp 342–354Google Scholar
  2. Aitken-Christie J, Kozai T, Takayama S. 1995. Automation in plant tissue culture: general introduction and overview. In: T Kozai, MAL Smith, J Aitken-Christie, eds., Automation and environmental control in plant tissue culture. Kluwer, Boston, pp 1–18Google Scholar
  3. Ascough GD, Fennell CW. 2004. The regulation of plant growth and development in liquid culture. South Afr. J. Bot. 70, 181–190CrossRefGoogle Scholar
  4. Berthouly M, Etienne H. 2005. Temporary immersion system: a new concept for use liquid medium in mass propagation. In: AK Hvoslef-Eide, W Preil, eds., Liquid Culture Systems for In Vitro Plant Propagation. Springer, Dordrecht pp 165–195Google Scholar
  5. Cai Lin, Lin Fu, Ji L. 2011. Regeneration of Jatropha curcas through efficient somatic embryogenesis and suspension culture. GM Crops 2: 110–117CrossRefPubMedGoogle Scholar
  6. De Klerk, Ter Brugge J. 2011. Micropropagation of dahlia in static liquid medium using slow-release tools of medium ingredients. Sci. Hort. 127: 542–547CrossRefGoogle Scholar
  7. Demissie AG, Lele SS. 2013. Determination of polyunsaturated fatty acids in Jatropha curcas somatic embryos and the effect of abiotic sources. Biol. Sci. Pharm. Res. 1: 8–15Google Scholar
  8. Deore AC, Johnson TS. 2008. High-frequency plant regeneration from leaf-disc cultures of Jatropha curcas L. an important biodiesel plant. Plant Biotechnol. Rep. 2: 10–15Google Scholar
  9. Etienne H, Dechamp E, Barry-Etienne D, Bertrand B. 2006. Bioreactors in coffee micropropagation. Brazil J. Plant Physiol. 18: 45–54CrossRefGoogle Scholar
  10. Guan H, De Klerk GJ. 2000. Stem segments of apple microcuttings take up auxin predominantly via the cut surface and not via the epidermal surface. Sci. Hort. 86: 23–32CrossRefGoogle Scholar
  11. Ivanova M, Van Staden J. 2011. Influence of gelling agent and cytokinins on the control of hyperhydricity in Aloe olyphylla. Plant Cell Tiss. Organ Cult. 104: 13–21CrossRefGoogle Scholar
  12. Khemkladngoen N, Cartagena J, Shibagaki N, Fukui K. 2011. Adventitious shoot regeneration from juvenile cotyledons of a biodiesel producing plant Jatropha curcas L. J. Biosci. Bioeng. 111: 67–70CrossRefPubMedGoogle Scholar
  13. Khurana-Kaul V, Kachhwaha S, Kothari SL. 2010. Direct shoot regeneration from leaf explants of Jatropha curcas in response to thidiazuron and high copper contents in the medium. Biol. Plant. 54: 369–372CrossRefGoogle Scholar
  14. Kumar N, Reddy MP. 2010. Plant regeneration through the direct induction of shoot buds from petiole explants of Jatropha curcas: a biofuel plant. Annu. Appl. Biol. 156: 367–375CrossRefGoogle Scholar
  15. Kumar N, Reddy MP. 2012. Thidiazuron (TDZ) induced plant regeneration from cotyledonary petiole explants of elite genotypes of Jatropha curcas: a candidate biodiesel plant. Ind. Crops Prod. 39: 62–68CrossRefGoogle Scholar
  16. Kumar N, Vijayanand KG, Reddy MP. 2011. In vitro regeneration from petiole explants of non-toxic Jatropha curcas. Ind. Crops Prod. 33: 146–151CrossRefGoogle Scholar
  17. Loberant B, Altman A. 2010. Micropropagation of plants. In: MC Flickinger, ed., Encyclopedia of Industrial Biotechnology: Biprocess, Biosepar Cell Tech Wiley, New York, pp 3499–3515Google Scholar
  18. Mujib A, Muzamil A, Tasiu I, Dipti H. 2014. Somatic embryo mediated mass production of Catharanthus roseus in culture vessel (bioreactor) -A comparative study. Saudi J. Biol. Sci. 21: 442–449CrossRefPubMedPubMedCentralGoogle Scholar
  19. Navarro-Pineda FS, Baz-Rodríguez SA, Handler R, Sacramento-Rivero JC. 2016. Advances on the processing of Jatropha curcas towards a whole-crop biorefinery. Renew Sustain Energy Rev. 54: 247–269CrossRefGoogle Scholar
  20. Niemenak N, Saare-Surminski K, Rohsius C, Ndoumou DO, Lieberei R. 2008. Regeneration of somatic embryos in Theobroma cacao L. in temporary immersion bioreactor and analyses of free amino acids in different tissues. Plant Cell Rep. 27: 667–676PubMedGoogle Scholar
  21. Openshaw K. 2000. A review of Jatropha curcas: an oil plant of unfulfilled promise Biomass Bioenergy 19: 1–15Google Scholar
  22. Paek KY, Chakrabarty D, Hahn EJ. 2005. Application of bioreactor systems for large scale production of horticultural and medicinal plants. Plant Cell Tiss. Organ Cult. 81: 287–300CrossRefGoogle Scholar
  23. Paek KY, Hahn EJ, Son SH. 2001. Application of bioreactors of large scale micropropagation systems of plants. In Vitro Cell. Dev. Biol. Plant 37: 149–157CrossRefGoogle Scholar
  24. Piatczak E, Wielanek M, Wysokinska H. 2005. Liquid culture system for shoot multiplication and secoiridoid production in micropropagated plants of Centaurium erythraea Rafn. Plant Sci. 168: 431–437CrossRefGoogle Scholar
  25. Preil W. 2005. General introduction: a personal reflection on the use of liquid media for in vitro culture. In: W Preil, AK Hvoslef-Eide, eds., Liquid culture systems for in vitro plant propagation. Springer, Berlin, pp 1–18Google Scholar
  26. Quiala E, Barbon R, Jime´nez E, de Feria M, Cha´vez M, Capote A, Perez N. 2006. Biomass production of Cymbopogon citratus (DC) Stapf., a medicinal plant, in temporary immersion systems. In vitro Cell. Dev. Biol.-Plant 42: 98–300CrossRefGoogle Scholar
  27. Quiala E, Canal MJ, Meijon M, Rodriguez R, Chavez M, Valledor L, Feria M, Barbon A 2012. Morphological and physiological responses of proliferating shoots of teak to temporary immersion and BA treatments. Plant Cell Tiss. Organ Cult. 109: 223–234CrossRefGoogle Scholar
  28. Salaj T, Blehová A, Salaj J 2007. Embryogenic suspension cultures of Pinus nigra Arn.: growth parameters and maturation ability. Acta Physiol. Plant. 29: 225–231CrossRefGoogle Scholar
  29. Schönherr J. 2006. Characterization of aqueous pores in plant cuticles and permeation of ionic solutes. J. Exp. Bot. 57: 2471–2491CrossRefPubMedGoogle Scholar
  30. Shaik S, Dewir YH, Singh N, Nicholas A 2010. Micropropagation and bioreactor studies of the medicinally important plant Lessertia (Sutherlandia) frutescens L. South Afr. J. Bot. 76: 180–186CrossRefGoogle Scholar
  31. Singh A, Jani K, Sagervanshi A, Agrawal PK. 2014. High frequency regeneration by Abscisic Acid (ABA) from petiole callus of Jatropha curca.s In Vitro Cell. Dev. Biol. Plant 50: 638–645CrossRefGoogle Scholar
  32. Singh B, Yadav K, Lal M. 2001. An efficient protocol for micropropagation of sugarcane using shoot tip explants. Sugar Technol. 3: 113–116CrossRefGoogle Scholar
  33. Snyman SJ, Nkwanyana PD, Watt MP. 2011. Alleviation of hyperhydricity of sugarcane plantlets produced in RITA (R) vessels and genotypic and phenotypic characterization of acclimated plants. South Afr. J. 77: 685–692CrossRefGoogle Scholar
  34. Soomro R, Memon R. 2007. Establishment of callus and suspension culture in J. curcas. Pak. J. Bot. 39: 2431–2441Google Scholar
  35. Veltcheva MR, Svetleva DL. 2005. In vitro regeneration of Phaseolus vulgaris L. via organogenesis from petiole explants. J. Central Eur. Agric. 6: 53–58Google Scholar
  36. Wenck AR, Quinn M, Whetten, Pullman G, Sederoff R. 1999. Agrobacterium-mediated transformation of Norway spruce (Picea abies) and loblolly pine (Pinus taeda). Plant Mol. Biol. 39: 407–416CrossRefPubMedGoogle Scholar
  37. Ziv M. 2010. Bioreactor technology for plant micropropagation. In: J Janick, ed, Horticulture Reviews. Wiley, New York, pp 1–30Google Scholar

Copyright information

© Korean Society of Crop Science and Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Discipline of plant Omics, CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI), Council of Scientific & Industrial Research (CSIR)Gijubhai Badheka MargBhavnagarIndia

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