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Optimal protocol for mass propagation of Aloe vera

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Abstract

We established an advanced protocol for in vitro propagation of Aloe vera via comparison of basal media, sucrose contents, growth hormone combinations, and additional supplementation with various polyamines. The maximal number and growth of shoots after 5 weeks was obtained using MS media including 30 g L−1 sucrose supplemented with 1.0 mg L−1 BA and 0.1 mg L−1 NAA. To improve shoot production, various concentrations of putrescine, spermidine, and spermine were added under optimal growth hormone conditions (MS media supplemented with 30 g L−1 sucrose, 1.0 mg L−1 BA, and 0.1 mg L−1 NAA). Maximal shoot number and growth after 5 weeks were achieved with supplementation of 50 mg L−1 spermidine. Regenerated plants were successfully acclimatized in soil with 100% efficiency. Cytogenetic inspection revealed that the regenerated plants maintained intact chromosomes identical to those of plants grown in field conditions. This protocol provides a valuable alternative for mass production of elite Aloe vera.

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Abbreviations

BA:

6-benzylaminopurine

B5 media:

Gamborg B5 medium

MS media:

Murashige and Skoog media

NAA:

1-naphthalene acetic acid

SH media:

Schenk and Hildebrandt media

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Authors and Affiliations

  1. Department of Plant Science, Plant Genomics and Breeding Institute, and Research Institute for Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Korea

    Yun Sun Lee, Hye Mi Park & Tae-Jin Yang

  2. Division of Plant Science and Resources, Chungnam National University, Daejeon, 305-754, Korea

    Sang Un Park

  3. Kim Jeong Moon Aloe Co. LTD, 3rd Fl., BaekJae Bldg. 1580-10 SeoCho3-Dong, SeoCho-Gu, Seoul, 137-875, Korea

    Jin Hong Baek

Authors
  1. Yun Sun Lee
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  2. Hye Mi Park
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  3. Sang Un Park
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  4. Jin Hong Baek
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  5. Tae-Jin Yang
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Correspondence to Tae-Jin Yang.

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Lee, Y.S., Park, H.M., Park, S.U. et al. Optimal protocol for mass propagation of Aloe vera . J. Crop Sci. Biotechnol. 16, 285–290 (2013). https://doi.org/10.1007/s12892-013-0099-1

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  • DOI: https://doi.org/10.1007/s12892-013-0099-1

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