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Antimicrobial and micro Raman spectroscopy of selected Zingiberaceae species from Northeast India

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Abstract

Antimicrobial activity of crude rhizome oils from some important members of Zingiberaceae from Northeast India was carried out in the present study. The plants used were Curcuma amada, C. longa, Zingiber moran, and Z. zerumbet. Rhizomes of the four species were collected and rhizome oil was extracted using six different polar and non-polar solvents. The antimicrobial property of the crude oil was studied against human pathogens using disc-diffusion and viability assay. The rhizome oil of all the 24 extracts exhibited potent antimicrobial activity against all pathogenic bacterial and fungal strains tested. Hydrodistilled fractions of the four species studied showed better effect among all solvents. Water extract of Z. moran was found to be the highest effective antimicrobial agent of all. Antibacterial effect of the water fractions was also characterized against Gram-positive and Gram-negative bacteria using micro Raman spectroscopy. This investigation shows that the essential oils from the wild and domestic species of the family Zingiberaceae from Northeast India are a potential source of bioactive compounds with tremendous medicinal, economic, and therapeutic value. The wild and endemic species Z. moran represents an interesting and promising source of plant-derived antimicrobial agent in the development of new drugs against pathogens.

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Abbreviations

ANOVA:

analysis of variance

CDA:

Czapek Dox agar

DMSO:

dimethyl sulfoxide

LSD:

least significant difference

MIC:

minimal inhibitory concentration

PDA:

potato dextrose agar

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Authors and Affiliations

  1. Department of Biotechnology, Indian Institute of Technology Guwahati, North Guwahati, Assam, 781 039, India

    Archana Das, Vigya Kesari & Latha Rangan

  2. Department of Physics, Indian Institute of Technology Guwahati, North Guwahati, Assam, 781 039, India

    Arpita Nath & Alika Khare

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  1. Archana Das
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  2. Vigya Kesari
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  3. Arpita Nath
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  4. Alika Khare
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  5. Latha Rangan
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Correspondence to Latha Rangan.

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Das, A., Kesari, V., Nath, A. et al. Antimicrobial and micro Raman spectroscopy of selected Zingiberaceae species from Northeast India. J. Crop Sci. Biotechnol. 16, 75–81 (2013). https://doi.org/10.1007/s12892-011-0019-1

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  • DOI: https://doi.org/10.1007/s12892-011-0019-1

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