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Comparison and analysis of main effects, epistatic effects, and QTL × environment interactions of QTLs for agronomic traits using DH and RILs populations in rice

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Abstract

Two genetic linkage maps based on doubled haploid (DH) and recombinant inbred lines (RILs) populations, derived from the same indica-japonica cross ‘Samgang × Nagdong’, were constructed to analyze the quantitative trait loci (QTLs) affecting agronomic traits in rice. The segregations of agronomic traits in RILs population showed larger variations than those in DH population. A total of 10 and 12 QTLs were identified on six chromosomes using DH population and seven chromosomes using RILs population, respectively. Three stable QTLs including pl9.1, ph1.1, and gwp11.1 were detected through different years. The percentages of phenotypic variation explained by individual QTLs ranged from 8 to 18% in the DH population and 9 to 33% in the RILs population. Twenty-three epistatic QTLs were identified in the DH population, while 21 epistatic QTLs were detected in the RILs population. Epistatic interactions played an important role in controlling the agronomic traits genetically. Four significant main-effect QTLs were involved in the digenic interactions. Significant interactions between QTLs and environments (QE) were identified in two populations. The QTLs affecting grain weight per panicle (GWP) were more sensitive to the environmental changes. The comparison and QTLs analysis between two populations across different years should help rice breeders to comprehend the genetic mechanisms of quantitative traits and improve breeding programs in marker-assisted selection (MAS).

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Authors and Affiliations

  1. School of Applied Biosciences, Kyungpook National University, Daegu, 702-701, Korea

    Xinhua Zhao, Yang Qin, Suk-Man Kim, Hyun-Suk Lee, Moo-Young Eun & Jae-Keun Sohn

  2. Department of Agronomy, Shenyang Agricultural University, Shenyang, 110161, China

    Xinhua Zhao & Baoyan Jia

  3. School of Applied Ecological Resources, College of Ecology & Environmental Science, Kyungpook National University, Sanju, 742-711, Korea

    Kyung-Min Kim

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  1. Xinhua Zhao
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  2. Yang Qin
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Correspondence to Jae-Keun Sohn.

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Zhao, X., Qin, Y., Jia, B. et al. Comparison and analysis of main effects, epistatic effects, and QTL × environment interactions of QTLs for agronomic traits using DH and RILs populations in rice. J. Crop Sci. Biotechnol. 13, 235–241 (2010). https://doi.org/10.1007/s12892-010-0076-x

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  • DOI: https://doi.org/10.1007/s12892-010-0076-x

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