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Journal of Crop Science and Biotechnology

, Volume 13, Issue 1, pp 39–46 | Cite as

Oil biosynthesis and its related variables in developing seeds of mustard (Brassica juncea L.) as influenced by sulphur fertilization

  • Inayat S. FaziliEmail author
  • Muzain Masoodi
  • Saif Ahmad
  • Arshad Jamal
  • Jafar S. Khan
  • Malik Z. Abdin
Research Article

Abstract

The aim of this research was to study the effect of sulphur (S) fertilization on oil biosynthesis and its related variables at various stages of seed development, and to find possible explanations for increased oil content in the seeds of mustard (Brassica juncea L. Czern and Coss) due to S fertilization. Acetyl-CoA carboxylase activity and contents of oil, acetyl-CoA, soluble protein, total RNA, total sugar and sulphur were determined in the developing seeds of mustard grown in the field with sulphur (+S) and without sulphur (-S). The period between 10 to 30 days after flowering was observed as the active period of oil accumulation in the developing seeds of mustard. The accumulation of the oil was preceded by a marked rise in acetyl-CoA carboxylase activity and acetyl-CoA concentration, which declined rapidly when oil accumulation reached a plateau. Total sugar content decreased, while protein content increased during the active period of oil accumulation in the developing seeds (i.e. between 10–30 days after flowering). Sulphur fertilization significantly (P < 0.05) enhanced the oil accumulation in the developing seeds at all the growth stages. The increase in the oil content was 5–63% with S fertilization over the control treatment. Acetyl-CoA carboxylase activity and contents of acetyl-CoA, soluble protein, total RNA and sugar were significantly (P < 0.05) higher in the developing seeds of +S-treated plants compared to — S-treated plants. It is suggested that the increase in the oil content with sulphur fertilization may be associated with the increases in acetyl-CoA carboxylase activity through the enhancement of acetyl-CoA concentration. Further, the increased sugar content due to S fertilization provided enough carbon source and energy for oil biosynthesis.

Key words

Brassica juncea L. oil total sugar soluble protein sulphur 

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Copyright information

© Korean Society of Crop Science and Springer Netherlands 2010

Authors and Affiliations

  • Inayat S. Fazili
    • 1
    • 2
    Email author
  • Muzain Masoodi
    • 3
  • Saif Ahmad
    • 1
    • 4
  • Arshad Jamal
    • 1
    • 5
  • Jafar S. Khan
    • 6
  • Malik Z. Abdin
    • 1
  1. 1.Centre for Transgenic Plant Development, Department of Biotechnology, Faculty of ScienceHamdard UniversityNew DelhiIndia
  2. 2.Center of Animal BiotechnologySher-i-Kashmir University of Agricultural Sciences and Technology (S KUAST-K)Shuhama (Alusteng), SrinagarIndia
  3. 3.Department of HorticultureSKUAST(K)Shalimar, KashmirIndia
  4. 4.Centre for Vascular BiologyMedical College of GeorgiaAugustaUSA
  5. 5.Department of Horticultural SciencesYeungnam UniversityGyeonbukRepublic of Korea
  6. 6.Council of Scientific and Industrial Research (CSIR)Anusandhan Bhawan, Rafi Marg, New DelhiIndia

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