Abstract
We studied the role of methyl jasmonate (MeJA) in alleviating NaCl-induced salt stress on soybean growth and development in hydroponics medium. Soybean seedlings were exposed to 60 mM NaCl stress for 2 weeks, 24 h after the application of 20 and 30 µM MeJA. NaCl stress induced a significant reduction in plant growth, endogenous bioactive gibberellin (GA4), photosynthesis and transpiration rate, while a marked increase in the endogenous abscisic acid (ABA) and proline contents were recorded. MeJA application greatly mitigated the adverse effects of NaCl on soybean growth and endogenous hormones. MeJA significantly increased ABA levels, while the endogenous amount of GA4 was reduced by the application of NaCl. Our study revealed that MeJA counteracted the negative effects of NaCl stress on plant growth, chlorophyll content, leaf photosynthetic rate, leaf transpiration rate, and proline content.
Similar content being viewed by others
References
Abel GH, MacKenzie AJ. 1964. Salt tolerance of soybean varieties (Glycine max L. Merill) during germination and later growth. Crop Sci. 4: 157–161
Ahmad P, Jhon R. 2005. Effect of salt stress on growth and biochemical parameters of Pisum sativum L. Arc. Agro. Soil Sci. 51: 665–672
Al-Aghabary K, Zhu Z, Qinhua S. 2004. Influence of silicon supply on chlorophyll content, chlorophyll fluorescence, and antioxidative enzyme activities in tomato plants under salt stress. J. Plant Nut. 27: 2101–2115
Amzallag GN, Lerner HR, Poljakoff-Mayber A. 1990. Exogenous ABA as a modulator of the response of Sorghum to high salinity. J. Exp. Bot. 41: 1529–1534
Anuradha S, Rao SSR. 2003. Application of brassinosteroids to rice seeds (Oryza sativa L.) reduced the impact of salt stress on growth, prevented photosynthetic pigment loss and increased nitrate reductase activity. Plant Growth Reg. 40: 29–32
Aoki A, Kanegami A, Mihara M, Kojima T, Shiraiwa M, Takahara H. 2005. Molecular cloning and characterization of a novel soybean gene encoding a leucine-zipper-like protein induced to salt stress. Gene 356: 135–145
Ashraf M, Karim F, Rasul E. 2002. Interactive effects of gib berellic acid (GA3) and salt stress on growth, ion accumulation and photosynthetic capacity of two spring wheat (Triticum aestivum L.) cultivars differing in salt tolerance. Plant Growth Reg. 36: 49–59
Bates LS, Waldren RP, Teare ID. 1973. Rapid determination of free proline for water-stress studies. Plant Soil 39: 205–207
Blumwald E, Grober A. 2006. Salt tolerance, In NG Halford, ed, Plant Biotechnology: Current and future uses of genetically modified crops, John Wiley and Sons Ltd., UK, pp. 206–224
Boucaud J, Unger IA. 1976. Hormonal control of germination under saline conditions of three halophyte taxa in genus Suaeda. Physiol. Plant. 36: 197–200
Bohm W. 1979. Methods of studying root systems. Springer-Verlag, Berlin
Chao WS, Gu YQ, Pautot V, Bray EA, Walling LL. 1999. Leucine aminopeptidase RNAs, proteins, and activities increase in response to water deficit, salinity, and the wound signals systemin, methyl jasmonate, and abscisic acid. Plant Physiol. 120: 979–992
Chen THH, Gusta LV. 1983. Abscisic acid-inducing freezing resistance in cultured plant cells. Plant Physiol. 73: 71–75
Cheong JJ, Choi YD. 2003. Methyl jasmonate as a vital substance in plants. Trends Genet. 19: 409–413
Chon SU, Park JH, Choi WY, Jung SY. 2003. Differential physiological responses of soybean. Korea Soybean Digest 20: 17–27
Creelman RA, Rao MV. 2002. The oxylipin pathway in Arabidopsis, In CR Somervile, EM Meyerowitz, eds, The Arabidopsis Book., American Society of Plant Biologists, USA
Epstein E, Norlyn JD, Rush DW, Kings RW, Kelly DB, Cunningham DB, Worna AF. 1980. Saline culture of crops. A general approach. Sci. 210: 399–404
Essa TA. 2002. Effect of salinity stress on growth and nutrient composition of three Soybean (Glycine max L. Merrill) culti vars. J. Agro. Crop Sci. 188: 86–93
Fedina IS, Tsonev TD. 1997. Effect of pretreatment with methyl jasmonate on the response of Pisum sativum to salt stess. J. Plant Physiol. 151: 735–740
Flowers TJ, Yeo AR. 1995. Breeding for salinity resistance in crop plants: Where next? Aust. J. Plant Physiol. 22: 875–884
Gonzalez EM, Galvez L, Arrese-Igor C. 2001. Abscisic acid induces a decline in nitrogen fixation that involves leghemoglobin, but is independent of sucrose synthase activity. J. Exp. Bot. 52: 285–293
Hoyos M, Zhang SQ. 2000. Calcium-independent activation of salicylic acid-induced protein kinase and a 40-kilodalton protein kinase by hyper osmotic stress. Plant Physiol. 122: 1355–1363
Jin S, Chen CCS, Plant AL. 2000. Regulation by ABA of osmotic-stress-induced changes in protein synthesis in tomato roots. Plant Cell Environ. 23: 51–60
Kamboj JS, Browning G, Blake PS, Quinlan JD, Baker DA, Kamboj JS. 1999. GC-MS SIM analysis of abscisic acid and indole-3-acetic acid in shoot bark of apple root stocks. J. Plant Growth Reg. 28: 21–27
Kang DJ, Seo YJ, Lee JD, Ishii R, Kim KU, Shin DH, Park SK, Jang SW, Lee IJ. 2005. Jasmonic acid differentially affects growth, ion uptake and abscisic acid concentration in salttolerant and salt-sensitive rice cultivars. J. Agron. Crop Sci. 191: 273–282
Kaya CH, Kirnak H, Higgs K. 2001. Enhancement of growth and normal growth parameters by foliar application of potassium and phosphorus in tomato cultivars grown at high (NaCl) salinity. J. Plant Nut. 24: 357–367
Khadri M, Tejera NA, Lluch C. 2006. Alleviation of salt stress in common bean by exogenous abscisic acid supply. J. Plant Growth Reg. 25: 110–119
La Rosa DC, Hasegawa D, Rhodes D, Clithero MJ, Watad AEA, Bressan RA. 1987. Abscisic acid stimulated osmotic adjustment and involvement in adaptation of tobacco cells to NaCl. Plant Physiol. 85: 174–181
Lauchli A. 1984. Salt exclusion: an adaptation of legumes for crops and pastures under saline conditions, In RC Staples, GH Toeniessen, eds, Salinity tolerance in plants. Strategies for crop improvement. John Wiley and Sons, New York, pp. 171–187
Lee IJ, Foster KR, Morgan PW. 1998. Photoperiod control of gibberellin levels and flowering in Sorghum. Plant Physiol. 116: 1003–1010
Montero E, Cabot C, Barcelo J, Poschenrieder C. 1997. Endogenous abscisic acid levels are linked to decreased growth of bush bean plants treated with NaCl. Physiol. Plant. 101: 17–22
Murillo-Amador B, Yamada S, Yamaguchi T, Rueda-Puente E, Avila-Serrano N, Garcia-Hernandez JL, Lopez-Aguilar R, Troyo-Dieguez E, Nieto-Garibay A. 2007. Influence of calcium silicate on growth, physiological parameters and mineral nutrition in two legume species under salt stress. J. Agron. Crop Sci. 193: 413–421
Navarro JM, Martinez V, Carvajal M. 2000. Ammonium, bicarbonate and calcium effects on tomato plants grown under saline conditions. Plant Sci. 157: 89–96
Norlyn JD, Epstein E. 1984. Variability in salt tolerance of four triticale lines at germination and emergence. Crop Sci. 24: 1090–1992
Qi QG, Rose PA, Abrams GD, Taylor DC, Abrams SR, Cutler AJ. 1998. (+)-Abscisic acid metabolism, 3-ketoacyl-coenzyme A synthase gene expression, and very-long-chain monounsaturated fatty acid biosynthesis in Brassica napus embryos. Plant Physiol. 117: 979–987
Seo HS, Kim SK, Jang SW, Choo YS, Sohn EY, Lee IJ. 2005. Effect of jasmonic acid on endogenous gibberellins and abscisic acid in rice under NaCl stress. Biologia Plant. 49: 447–450
Serrano R, Gaxiola R. 1994. Microbial models and salt stress tolerance in plants. Crit. Rev. Plant Sci. 13: 121–138
Thaler JS. 1999. Jasmonate-inducible plant defenses cause increased parasitism of herbivores. Nature 399: 686–688
Velitcukova M, Fedina I. 1998. Response of photosynthesis of Pisum sativum to salt stress as affected by methyl jasmonate. Photosynthetica 35: 89–97
Wang Y, Mopper S, Hasenstein KH. 2001. Effects of salinity on endogenous ABA, IAA, JA and SA in Iris hexagona. J. Chem. Ecol. 27: 327–342
Wasternack C, Hause B. 2002. Jasmonates and octadecanoids: signals in plant stress responses and development. Prog. Nucleic Acid Res. Mol. Biol. 72: 165–221
Wasternack C, Parthier B. 1997. Jasmonate-signalled plant gene expression. Trends Plant Sci. 2: 302–307
Yeo AR, Flowers SA, Rao G, Welfare K, Senanayake N, Flowers TJ. 1999. Silicon reduces sodium uptake in rice (Oryza sativa L.) in saline conditions and this is accounted for by a reduction in the transpirational bypass flow. Plant Cell Environ. 22: 559–565
Yoon BS, Jin CJ, Un PS, Cho DH. 2005. Change in photosynthesis, proline content, and osmotic potential of Corn seedling under high-saline condition. Korean J. Crop Sci. 50: 28–31
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Yoon, J.Y., Hamayun, M., Lee, SK. et al. Methyl jasmonate alleviated salinity stress in soybean. J. Crop Sci. Biotechnol. 12, 63–68 (2009). https://doi.org/10.1007/s12892-009-0060-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12892-009-0060-5