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Isolation and characterization of 35 SNP markers of Leiobagrus kingi (Teleostei, Siluriformes: Amblycipitidae)

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Leiobagrus kingi is a species endemic to southwest China, it is listed as an endangered species. Due to habitat destruction, over-fishing and water pollution, the wild population of L. kingi has decreased dramatically in recent years. Insufficient molecular markers have limited the effective conservation of this species. In the present study, 35 novel single-nucleotide polymorphism (SNP) markers in L. kingi were isolated and characterized based on restriction site-associated DNA sequencing (RAD-seq). The minor allele frequency ranged from 0.043 to 0.481, and the observed and expected heterozygosities ranged from 0.077 to 0.678 and 0.105 to 0.505, respectively. Polymorphic information content ranged from 0.072 to 0.411. Among these SNPs, five loci showed significant departures from the Hardy–Weinberg equilibrium (P < 0.01). The novel polymorphic SNPs will be useful for future investigation of population structure and conservation genetics for this species.

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This study was supported by Laboratory of XianNing college.


The research funding was provided by the Xianning First People’s Hospital.

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XYJ conceived and designed the study, contributed to the collection of the samples and the drafted the manuscript. WL participated in the data collection, purification, and management of the samples for sequencing and genotyping. All authors have reviewed and approved the manuscript.

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Correspondence to Xiang-Yang Jia.

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Li, W., Jia, XY. Isolation and characterization of 35 SNP markers of Leiobagrus kingi (Teleostei, Siluriformes: Amblycipitidae). Conservation Genet Resour 16, 187–189 (2024).

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