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Isolation and characterization of 46 SNP markers in Gymnocypris chilianensis

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In the present study, we identified 46 SNP markers for Gymnocypris chilianensis, an endemic economic fish in Heihe River and Shule River in China. The minor allele frequency ranged from 0.028 to 0.500. Observed heterozygosity ranged from 0.028 to 1.000, and expected heterozygosity from 0.055 to 0.507. The polymorphic information content ranged between 0.053 and 0.375. Among these SNP markers, sixteen loci showed significant departures from Hardy-Weinberg equilibrium after Bonferroni correction. These novel SNP markers will be helpful for future studies on genetic management and population conservation in this species.

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Data Availability

The data and material that support the findings of this study are available from the corresponding author upon reasonable request.

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The authors are grateful to Han Zhao, Zhongmeng Zhao and Hongyu Ke for their help in sample collection.


This work was supported by grants from the Project of Investigation on Fishery Resources and Environment of Northwest China, the Project of Sichuan Science and Technology Program (2021YFYZ0015).

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Authors and Affiliations



Ya Liu designed the experiment and wrote the main manuscript. Wenqiong Wu and Siyi Zhu performed the experiments. Yeyu Chen prepared Table 1. Xiaoyun Wu checked the data. Jiansheng Lai, Mingjiang Song and Feiyang Li provided formal analysis. Qiang Li provided the samples and revised the manuscript. All authors read and approved the final manuscript.

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Correspondence to Qiang Li.

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All fish handling and experimental procedures were approved by Animal Care and Use Committee of the Fishery Institute of the Sichuan Academy of Agricultural Sciences, and all animal collection and use protocols were carried out in accordance with the guidelines and regulations for the care and use of laboratory animals at the Fishery Institute of the Sichuan Academy of Agricultural Sciences.

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Liu, Y., Wu, W., Zhu, S. et al. Isolation and characterization of 46 SNP markers in Gymnocypris chilianensis. Conservation Genet Resour 15, 77–81 (2023).

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