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Table 1 The workflow for the detection of amphibian species based on metabarcoding of eDNA from water samples can identify all pond-dwelling amphibians of Switzerland and adjacent regions as given in the table (the only exception is Bufotes viridis which is extinct in Switzerland)

From: Ready-to-use workflows for the implementation of genetic tools in conservation management

Ichthyosaura alpestris

Lissotriton helveticus

Lissotriton vulgaris

Triturus cristatus

Triturus carnifex

Alytes obstetricans

Bombina variegata

Bufo bufo

Epidalea calamita

Hyla arborea

Hyla intermedia

Pelophylax bedriagae

Pelophylax bergeri

Pelophylax kurtmuelleri/P. ridibundus

Pelophylax esculentus/P. lessonae

Rana dalmatina

Rana latastei

Rana temporaria

  1. Especially, the workflow is able to discriminate between species or species groups of water frogs (Pelophylax sp.) or closely related species (e.g. Triturus cristatus and T. carnifex)
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