Abstract
The phenotypic identification of sex in Sebastes rockfish is difficult and often impractical from a management perspective, and the genetic basis of sex determination in the genus is currently uncertain. We tested a previously developed sex identification polymerase chain reaction restriction fragment length polymorphism (PCR–RFLP) assay in eight species of Sebastes rockfish. Results indicated that the association of this restriction site with sex is species-dependent.
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References
Anderson KL (1979) A karyological investigation of the systematics of the genus Sebastes (subgenus Pteropodus). Thesis, California State University, San Jose
Andrews KS, Nichols KM, Elz A, Tolimieri N, Harvey CJ, Pacunski R, Lowry D, Yamanaka KL, Tonnes DM (2018) Cooperative research sheds light on population structure and listing status of threatened and endangered rockfish species. Conserv Genet 19:865–878. https://doi.org/10.1007/s10592-018-1060-0
Berkeley SA (2006) Pacific rockfish management: are we circling the wagons around the wrong paradigm? Bull Mar Sci 78:655–667
Berkeley SA, Chapman C, Sogard SM (2004a) Maternal age as a determinant of larval growth and survival in a marine fish, Sebastes melanops. Ecology 85:1258–1264. https://doi.org/10.1890/03-0706
Berkeley SA, Hixon MA, Larson RJ, Love MS (2004b) Fisheries sustainability via protection of age structure and spatial distribution of fish populations. Fisheries 29:23–32. https://doi.org/10.1577/1548-8446(2004)29[23:FSVPOA]2.0.CO;2
Charlesworth D, Mank JE (2010) The birds and the bees and the flowers and the trees: lessons from genetic mapping of sex determination in plants and animals. Genetics 186:9–31. https://doi.org/10.1534/genetics.110.117697
Fowler BL, Buonaccorsi VP (2016) Genomic characterization of sex-identification markers in Sebastes carnatus and Sebastes chrysomelas rockfishes. Mol Ecol 25:2165–2175. https://doi.org/10.1111/mec.13594
Frable BW, Wagman DW, Frierson TN, Aguilar A, Sidlauskas BL (2015) A new species of Sebastes (Scorpaeniformes: Sebastidae) from the northeastern Pacific, with a redescription of the blue rockfish, S. mystinus (Jordan and Gilbert, 1881). Fish Bill 113:355–377. https://doi.org/10.7755/FB.113.4.1
Hanson KC, Gravel MA, Graham A, Shoji A, Cooke SJ (2008) Sexual variation in fisheries research and management: when does sex mater? Rev Fish Sci 16:421–436. https://doi.org/10.1080/10641260802013866
Hixon MA, Johnson DW, Sogard SM (2014) BOFFFFs: on the importance of conserving old-growth age structure in fishery populations. ICES J Mar Sci 71:2171–2185. https://doi.org/10.1093/icesjms/fst200
Hyde JR, Vetter RD (2007) The origin, evolution, and diversification of rockfishes of the genus Sebastes (Cuvier). Mol Phylogenet Evol 44:790–811. https://doi.org/10.1016/j.ympev.2006.12.026
Ida H, Iwasawa T, Kamitori M (1982) Karyotypes in Eight Species of Sebastes from Japan. Jap J Ichthyol 29:162–168
Love MS, Yaklovich M, Thorsteinson L (2002) The rockfishes of the Northeast Pacific. University of California Press, Berkeley
Lowry DB, Hoban S, Kelley JL, Letterhos KE, Reed LK, Antolin MF, Storfer A (2017) Breaking RAD: an evaluation of the utility of restriction site-associated DNA sequencing for genome scans of adaptation. Mol Ecol Resour 17:142–152. https://doi.org/10.1111/1755-0998.12635
PFMC (Pacific Fishery Management Council) (2016) Pacific Coast Groundfish Fishery Management Plan for the California, Oregon, and Washington Groundfish Fishery. National Oceanographic and Atmospheric Administration, Silver Spring, MD. https://www.pcouncil.org/groundfish/fishery-management-plan/
Vaux F, Rasmuson LK, Kautzi LA, Rankin PS, Blume MTO, Lawrence KA, Bohn S, O’Malley KG (2019) Sex matters: otolith shape and genomic variation in deacon rockfish (Sebastes diaconus). Ecol Evol 9:13153–13173. https://doi.org/10.1002/ece3.5763
Worton CL, Rosenkranz GE (2003) Sex, age, and growth of black rockfish Sebastes melanops from a newly exploited population in the Gulf of Alaska, 1993–1999. Alaska Fish Res Bull 10:14–27
Acknowledgements
Samples from central Oregon were collected by Leif K. Rasmuson, Oregon Department of Fish and Wildlife. We thank the CPFV Enterprise for collecting the nearshore samples and the FV Last Straw for collecting the shelf samples. We are grateful to Vincent P. Buonaccorsi, Juniata College for sharing further samples and assay information. We thank the Editor in Chief, Benoit Goossens and an anonymous reviewer for their useful feedback on this manuscript.
Funding
This research was funded by the National Science Foundation Research Experience for Undergraduates (NSF REU) in Division of Ocean Sciences, co-funded by the ASSURE program of the Department of Defense in partnership with the National Science Foundation REU Site program under Grant No. NSF OCE-1758000.
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FV and HA wrote most of the manuscript, and all authors contributed to drafts. LR collected and identified the sex of samples. HA and SB conducted the laboratory and bioinformatic work. FV and KO co-supervised the project, and KO funded the project.
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Vaux, F., Aycock, H.M., Bohn, S. et al. Sex identification PCR–RFLP assay tested in eight species of Sebastes rockfish. Conservation Genet Resour 12, 541–544 (2020). https://doi.org/10.1007/s12686-020-01150-y
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DOI: https://doi.org/10.1007/s12686-020-01150-y