Abstract
Prunus mira, an important economic fruit crop and ancestral peach species, is widely distributed along the Yarlung Zangbo Grand Canyon and tributary basins in the Tibetan plateau. Due to infrastructure construction and deforestation, natural habitat of P. mira has been severely damaged, leading to reduction in its distribution range and population size and it is listed as a critically endangered species in China’s list of National Key Protected Wild Plants. In this study, 85 novel single nucleotide polymorphism (SNP) markers were developed based on restriction-site associated DNA sequencing. The minor allele frequency, observed heterozygosity, and expected heterozygosity ranged from 0.064 to 0.748, 0.086 to 0.846, and 0.126 to 0.562, respectively. The PIC ranged from 0.117 to 0.423. Ten loci showed significant deviations from the Hardy–Weinberg equilibrium after Bonferroni correction (p < 0.05). These novel SNPs can be useful for future population and conservation genetics studies on this species.
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Acknowledgements
This project was supported by the Research Fund for Young Teachers of the College of Forestry of Inner Mongolia Agricultural University (Grant Number 203-170007), High-level Talent Introduction Project of Inner Mongolia Agricultural University (Grant Number NDYB2017-9), Natural Science Foundation of Inner Mongolia Autonomous Region (Grant Number 2019BS03019), and the National Natural Key R&D Program of China (Grant Number 2018YFD1000606-3).
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Bao, W., Ao, D., Wuyun, T. et al. Development of 85 SNP markers for the endangered plant species Prunus mira (Rosaceae) based on restriction site-associated DNA sequencing (RAD-seq). Conservation Genet Resour 12, 525–527 (2020). https://doi.org/10.1007/s12686-020-01140-0
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DOI: https://doi.org/10.1007/s12686-020-01140-0