Abstract
The vulnerable African timber species Nauclea diderrichii is threatened because of its overexploitation and illegal trade. We sampled individuals across Ghana, Nigeria, Cameroon, Congo Brazzaville, Gabon and Democratic Republic of Congo and performed restriction associated DNA sequencing and low coverage MiSeq genome sequencing to discover SNP and INDEL loci. After MassARRAY genotyping of 95 individuals covering the sampled distribution range at 205 loci, a set of 88 loci were selected according to their differentiation among geographical regions. It is the first time that molecular markers are described for N. diderrichii and it should encourage genetic studies in this valuable species.
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Acknowledgements
This research was supported by the German Federal Ministry of Food and Agriculture in the frame of the “Large scale project on genetic timber verification” (Project No. 28I-001-01). Genotyping was performed at the Genomic and Sequencing Facility of Bordeaux (grants from the Conseil Regional d’Aquitaine no. 20030304002FA and 20040305003FA, the European Union, FEDER no. 2003227 and Investissements d’avenir, no. ANR-10-EQPX-16-01 and CEBA: ANR-10-LABX-25-01 to the UMR EcoFoG). Sampling was facilitated by the help of the CNIAF of republic of Congo, the MINFOF of Cameroon, the Tshopo provincial ministry in charge of environment, the Institut Supérieur des Etudes Agronomiques de Bengamisa (ISEA), the Institut Faculté des Sciences Agronomiques de Yangambi (IFA/Yangambi) of DRC, the Forestry Commission of Ghana, the SODEFOR of Cote d’Ivoire, the Liberia Forest Authority and the Nigeria Forest Service. We are grateful to Susanne Bein and Maike Paulini for the work conducted in the laboratory, Lennart Meyer-Sand and Birte Pakul for their assistance in sampling organisation as well as sample preparation and documentation.
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Blanc-Jolivet, C., Mader, M., Bouda, HN. et al. Development of SNP markers for the African timber species Nauclea diderrichii. Conservation Genet Resour 12, 357–359 (2020). https://doi.org/10.1007/s12686-019-01115-w
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DOI: https://doi.org/10.1007/s12686-019-01115-w