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SNP marker development and polymorphism detection of Schizothorax waltoni

  • Jiejie Ma
  • Renxiao Zhang
  • Wei Zhao
  • Li Zhao
  • Mingrui Zhou
  • Tingsen Jing
  • Hui LuoEmail author
Technical Note
  • 35 Downloads

Abstract

As a vulnerable species in the Red List of China’s Vertebrates, Schizothorax waltoni (S. waltoni) is mainly distributed in the middle reaches of Yarlung Tsangpo River in Tibet. Due to its delicate meat and delicious taste, S. waltoni is the main economic fish in the producing area. But recently, it has undergone a drastic population decline due to the overfishing of fishery resources, the invasion of foreign species, environmental pollution and other factors. Therefore, it is extremely urgent to protect germplasm resource of S. waltoni. In this study, we used Illumina Hiseq 4000 sequencing to develop single nucleotide polymorphism (SNP) markers in S. waltoni. Based on a large number of transcriptome sequences and candidate SNP loci obtained by previous transcriptome studies, 63 SNPs were randomly selected from immune-related genes and 36 loci exhibited significant polymorphisms in genotyping by Sequenom MassARRAY. The observed heterozygosity and expected heterozygosity varied from 0.0789 to 0.9577 and 0.0763 to 0.5033, respectively. There is only 5 SNP loci accord with Hardy–Weinberg equilibrium (p > 0.05) and no linkage disequilibria. These polymorphic SNP markers could be used for conservation genetics research in S. waltoni, and may provide new insights into future conservation strategies.

Keywords

Schizothorax waltoni Single nucleotide polymorphism (SNP) Sequenom MassARRAY 

Notes

Acknowledgements

This research was supported by the Fundamental Research Funds for the Central Universities (XDJK2015C034, XDJK2017C035, 5360300098).

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.College of Animal ScienceSouthwest UniversityChongqingPeople’s Republic of China
  2. 2.Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education)Key Laboratory of Aquatic Science of ChongqingChongqingPeople’s Republic of China

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