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Isolation and characterization of 40 SNP in largemouth bass (Micropterus salmoides)

  • Jiajia FanEmail author
  • Junjie Bai
  • Dongmei Ma
Technical Note

Abstract

The largemouth bass Micropterus salmoides was introduced into Guandong province of China in 1983, and is now one of the China’s primary cultured fish species. After a long period of farming, the populations are facing the stringent challenges of germplasm resources degraded, and genetic improvement is urgently needed. Thus, developing sufficient molecular markers for the conservation and molecular-aided selection of largemouth bass is urgent. In this study, we implemented restriction site associated DNA sequencing (RAD-seq) to develop single nucleotide polymorphism (SNP) markers. In total, 40 novel SNP markers were found to be polymorphic and showed bi-allelic in 49 individuals of largemouth bass. Those novel SNP markers may be useful for further genetic improvement of largemouth bass.

Keywords

Micropterus salmoides RAD-seq SNP Genetic improvement 

Notes

Acknowledgements

This work was supported by the Basic Research Business Fees of Chinese Academy of Fishery Sciences (2018SJ-YZ03); Natural Science Foundation of Guangdong (2016A030313148); and China Agriculture Research System (CARS-46-03).

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture, Pearl River Fisheries Research InstituteChinese Academy of Fishery SciencesGuangzhouChina

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