Characterization of the complete chloroplast genome of Cephalotaxus sinensis (Cupressales: Taxaceae), an endangered Tertiary relict tree endemic to China

  • Ren-Yan Duan
  • Li-Min Yang
  • Gan-Lin Wu
Technical Note


Cephalotaxus sinensis is an endangered Tertiary relict tree endemic to China with high medicinal values. To contribute to its conservation genetics studies, its complete chloroplast (cp) genome was assembled using Illumina high-throughput sequencing technology in this study. The cp genome is 135,646 bp long with an asymmetric base composition (32.5% A, 17.6% C, 17.5% G and 32.4% T). It encodes a set of 113 unique genes (incl. 82 protein coding, 27 tRNA and 4 rRNA genes) with one of them (trnQ-UUG) being duplicated. Besides, 14 genes (atpF, ndhA, ndhB, petB, petD, rpl2, rpl16, rpoC1, rps12, rps16, trnA-UGC, trnG-UCC, trnI-GAU and trnK-UUU) possess a single intron, and another gene (ycf3) has a couple of introns. Phylogenetic analysis generally supported the current family-level taxonomy of the order Cupressales, and suggested that C. sinensis is closely related to the congeneric C. oliveri.


Cephalotaxus sinensis Chloroplast genome MITObim Phylogenetic analysis 



This study was financially supported by grants from the Anhui Provincial Natural Science Foundation (1608085MC63) and Anhui Provincial Key Project of Higher Education Institutions for Excellent Visiting Scholar (gxfxZD2016172).

Supplementary material

12686_2018_1033_MOESM1_ESM.doc (106 kb)
Supplementary material 1 (DOC 105 KB)


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© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of Life SciencesAnqing Normal UniversityAnqingPeople’s Republic of China
  2. 2.Anhui Key Laboratory for Biodiversity Studies and Ecological Conservation in Southwestern Anhui ProvinceAnqingPeople’s Republic of China

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