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Characterization of the complete mitochondrial genome and phylogenetic analysis of Pelodiscus sinensis, a mutant Chinese soft-shell turtle

  • Jun Zhang
  • Qingyan Zhou
  • Xiaoli Yang
  • Peng Yu
  • Weishang Zhou
  • Yuan Gui
  • Xia Ouyang
  • Quan Wan
Technical Note

Abstract

The Chinese soft-shell turtle (Pelodiscus sinensis, Testudines: Pelodiscus) shows geographical variation, and one strain is the inked turtle. Wild population numbers have dropped substantially during the past decades, and the species is now classed as vulnerable. However, little genetic data exists so this study aimed to sequence and analyze the complete mitochondrial genome. The circular double-stranded genome is 17,145 bp in length and contains 13 protein-coding genes (PCGs), two rRNA genes, 22 tRNA genes, an L-strand replication origin and a control region. The base composition is 35.5% A, 27.3% T, 11.8% G and 25.4% C, with an AT content of 62.8%. Trionychidae species were divided into two clades based on phylogenetic analysis, and the closest genetic distance was between Trionyx axenaria and P. sinensis. This study provides basic genetic data for future studies on conservation biology, phylogenetic and evolutionary analysis of this inked strain of the Chinese soft-shell turtle.

Keywords

Pelodiscus sinensis Soft-shell turtle Mitochondrial genome Phylogeny 

Notes

Acknowledgements

This work was supported by the Anhui Natural Science Foundation of the Colleges and Universities (Grant No. KJ2016A241), the Open Project of National Key Laboratory of the Ecological and Biological Technology for Institute of Hydrobiology of Chinese Academy of Sciences (Grant No. 2016FB16), and the Key Youth Fund of Anhui Agricultural University (Grant No. 2015ZD05).

Compliance with ethical standards

Conflict of interest

The authors report no conflicts of interest. The authors are responsible for the content and writing of the paper.

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of Animal Science and TechnologyAnhui Agricultural UniversityHefeiChina
  2. 2.State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of HydrobiologyChinese Academy of SciencesWuhanChina
  3. 3.University of Chinese Academy of SciencesBeijingChina

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