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The complete chloroplast genome of the rare and endangered herb Paphiopedilum dianthum (Asparagales: Orchidaceae)

  • Na Hou
  • Gang Wang
  • Yayan Zhu
  • Lianhui Wang
  • Jiajuan Xu
Technical Note
  • 38 Downloads

Abstract

Paphiopedilum dianthum is a rare and endangered herb with restricted and fragmented populations. To contribute to its conservation, its complete chloroplast genome was assembled and annotated in the present study. The circular genome is 154,699 bp in length with an overall A+T content of 64.1%, and exhibits a typical quadripartite structure of the large (LSC, 86,861 bp) and small (SSC, 2416 bp) single-copy regions, separated by a pair of inverted repeat regions (IRs, 32,711 bp each). A total of 101 gene species were annotated, including 67 protein-coding (PCG), 30 tRNA and 4 rRNA gene species. Twenty-one gene species are duplicated within the IR regions, and 15 gene species contain one or two introns. Besides, five unique pseudogenes were detected. Phylogenetic analysis corroborated the current taxonomy of the family Orchidaceae at the subfamilial level, and indicated that P. dianthum is phylogenetically closely related to the congeneric P. niveum and P. armeniacum.

Keywords

Double flowered paphiopedilum Endangered species High-throughput sequencing MITObim Plastid genome 

Notes

Acknowledgements

The work was supported by the Department of Science & Technology of Guizhou Province (SY[2015]3019; NY[2010]3029; NY[2006]3062; [2008-01]; [2016]5711).

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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  • Na Hou
    • 1
  • Gang Wang
    • 1
  • Yayan Zhu
    • 1
  • Lianhui Wang
    • 1
  • Jiajuan Xu
    • 1
  1. 1.Guizhou Academy of ForestryGuiyangPeople’s Republic of China

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