Abstract
Aconitum carmichaelii: is a species of perennial herb with high medicinal and ornamental values, and needs urgent conservation. To facilitate its conservation genetics studies, its complete chloroplast genome was assembled from Illumina sequencing reads. The circular genome is 155,737 bp long, and comprises a pair of inverted repeat (IR) regions of 26,193 bp each, separated by a large single-copy (LSC) region of 86,330 bp and a small single-copy (SSC) region of 17,021 bp. It encodes 112 gene species (incl. 78 PCGs, 30 tRNAs & 4 rRNAs), with 18 of them being duplicated. Besides, 15 gene species possess a single intron, and another two gene species contain a couple of introns. The nucleotide composition is asymmetric with an overall A+T content of 61.9%. Phylogenetic analysis challenged the current taxonomy of the family Berberidaceae, and suggested A. carmichaelii as being closely related to the two congeners A. barbatum and A. chiisanense.
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Cai S-Z, Li J, Pan Y-Z, Chen Q-B, Jiang B-B, Ye C (2011) Effects of different plant densities on growth and development of Aconitum carmichaeli. Acta Pratacult Sin 20:278–282
Chen X, Li Q, Li Y, Qian J, Han J (2015) Chloroplast genome of Aconitum barbatum var. puberulum (Ranunculaceae) derived from CCS reads using the PacBio RS platform. Front Plant Sci 6:42. doi:10.3389/fpls.2015.00042
Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15
Hahn C, Bachmann L, Chevreux B (2013) Reconstructing mitochondrial genomes directly from genomic next-generation sequencing reads—a baiting and iterative mapping approach. Nucl Acids Res 41:e129. doi:10.1093/nar/gkt371
Hou D-B, Ren Z-L, Shu G-M (2006) RAPD analysis of genetic diversity in the wild populations of Aconitum carmichaeli Dexb. (Ranunculaceae) in China. Acta Ecol Sin 26:1833–1841. doi:10.3321/j.issn:1000-0933.2006.06.026
Hu P, Xia Y-L, Yang Y-X, Zhou X-J, Shu G-M (2004) Genetic diversity among Aconitum carmichaeli Debx. germplasm based on RAMP marker. Southwest Chin J Agric Sci 27:984–990. doi:10.16213/j.cnki.scjas.2014.03.036
Kim Y-K, Park C-w, Kim K-J (2009) Complete chloroplast DNA sequence from a Korean endemic genus, Megaleranthis saniculifolia, and its evolutionary implications. Mol Cells 27:365–381. doi:10.1007/s10059-009-0047-6
Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874. doi:10.1093/molbev/msw054
Li L, Yuichi K (2001) Aconitum Linnaeus. Flora of China 6:149–222
Lohse M, Drechsel O, Kahlau S, Bock R (2013) OrganellarGenomeDRAW—a suite of tools for generating physical maps of plastid and mitochondrial genomes and visualizing expression data sets. Nucl Acids Res 41:W575–W581. doi:10.1093/nar/gkt289
Murayama M, Mori T, Bando H, Amiya T (1991) Studies on the constituents of Aconitum species. IX. The pharmacological properties of pyro-type aconitine alkaloids, components of processed aconite powder ‘Kako-bushi-matsu’: analgesic, antiinflammatory and acute toxic activities. J Ethnopharmacol 35:159–164. doi:10.1016/0378-8741(91)90068-O
Park KT, Park S, (2016) Complete chloroplast genome of Clematis fusca var. coreana (Ranunculaceae ). Mitochondr DNA Part A 27:4056–4058. doi:10.3109/19401736.2014.1003841
Raubeson LA, Peery R, Chumley TW, Dziubek C, Fourcade HM, Boore JL, Jansen RK (2007) Comparative chloroplast genomics: analyses including new sequences from the angiosperms Nuphar advena and Ranunculus macranthus. BMC Genomics 8:174. doi:10.1186/1471-2164-8-174
Tian M-L, Tian Y-Q, Liu F, Huang Y-B (2007) The genetic diversity of germplasms of Aconitum carmichaeli Debx. revealed by RAPD markers. J Sichuan Agric Univ 25:63–67. doi:10.3969/j.issn.1000-2650.2007.01.013
Tong P, Wu C, Wang X, Hu H, Jin H, Li C, Zhu Y, Shan L, Xiao L (2013) Development and assessment of a complete-detoxication strategy for Fuzi (lateral root of Aconitum carmichaeli) and its application in rheumatoid arthritis therapy. J Ethnopharmacol 146:562–571. doi:10.1016/j.jep.2013.01.025
Zhou X-J, xia Y-L, Zhang M, Chen T-Z, Fang Q-M, Ding J, Shu G-M (2011) Resource survey of Aconitum carmichaeli plant in Sichuan Province. Resour Dev Mark 27:448–450. doi:10.3969/j.issn.1005-8141.2011.05.020
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This study was supported by grants from the Program for Innovative Research Team in IMPLAD (PIRTI-IT 1302).
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Yang, J., Zeng, X. & Guo, S. Characterization of the complete chloroplast genome of the perennial herb Aconitum carmichaelii (Ranunculales: Ranunculaceae). Conservation Genet Resour 10, 605–608 (2018). https://doi.org/10.1007/s12686-017-0875-1
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DOI: https://doi.org/10.1007/s12686-017-0875-1