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The complete chloroplast genome sequence of Actinidia kolomikta from north China

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Abstract

The complete chloroplast genome sequence of a wild kiwifruit species from north China, Actinidia kolomikta, was determined and mapped based on Illumina sequencing data. The complete chloroplast genome is 156,875 bp and comprises a pair of inverted repeat regions of 23,918 bp each, a large single-copy region of 88,568 bp, and a small single-copy region of 20,471 bp. The genome harbors 132 genes, including 85 protein-coding genes, 8 ribosomal RNA genes, and 39 transfer RNA genes. Compared with most other angiosperms, A. kolomikta has lost its clpP gene. Phylogenetic analysis based on chloroplast genomes indicates that A. kolomikta is closely related to Actinidia argute.

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References

  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215(3):403–410

    Article  PubMed  CAS  Google Scholar 

  • Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M, Kulikov AS, Lesin VM, Nikolenko SI, Pham S, Prjibelski AD, Pyshkin AV (2012) SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 19:455–477

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Cipriani G, Testolin R. Morgante M (1995) Paternal inheritance of plastids in interspecific hybrids of the genus Actinidia revealed by PCR-amplification of chloroplast DNA fragments. Molec Gen Genet 247:693–697. doi:10.1007/BF00290400

    Article  PubMed  CAS  Google Scholar 

  • Cipriani G, Testolin R, Gardner R (1998) Restriction-site variation of PCR-amplified chloroplast DNA regions and its implication for the evolution and taxonomy of Actinidia. J Theor Appl Genet 96: 389–396. doi:10.1007/s001220050754

    Article  CAS  Google Scholar 

  • Li D, Qi X, Li X, Li L, Zhong C, Huang H (2013a) Maternal inheritance of mitochondrial genomes and complex inheritance of chloroplast genomes in Actinidia Lind.: evidences from interspecific crosses. Mol Genet Genomics 288:101–110. doi:10.1007/s00438-012-0732-6

    Article  PubMed  CAS  Google Scholar 

  • Li J, Wang S, Yu J, Wang L, Zhou L (2013b). A modified CTAB protocol for plant DNA extraction. Chin Bull Bot 48:72–78

    Article  CAS  Google Scholar 

  • Luo R, Liu B, Xie Y, Li Z, Huang W, Yuan J, He G, Chen Y, Pan Q, Liu Y, Tang J, Wu G, Zhang H, Shi Y, Liu Y, Yu C, Wang B, Lu Y, Han C, Cheung DW, Yiu SM, Peng S, Zhu X, Liu G, Liao X, Li Y, Yang H, Wang J, Lam TW, Wang J (2012) SOAPdenovo2: an empirically improved memory-efficient short-read de novo assembler. Gigascience 1(1):1

    Article  Google Scholar 

  • Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729. doi:10.1093/molbev/mst197

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Testolin R, Cipriani G (1997) Paternal inheritance of chloroplast DNA and maternal inheritance of mitochondrial DNA in the genus Actinidia. Theor Appl Genet 94:897–903. doi:10.1007/s001220050493

    Article  CAS  Google Scholar 

  • Wyman SK, Jansen RK, Boore JL (2004) Automatic annotation of organellar genomes with DOGMA. Bioinformatics 20:3252–3255

    Article  PubMed  CAS  Google Scholar 

  • Yao X, Tang P, Li Z, Li D, Liu Y, Huang H (2015) The first complete chloroplast genome sequences in Actinidiaceae: genome structure and comparative Analysis. PLoS ONE 10(6):e0129347. doi: 10.1371/journal.pone.0129347

    Article  PubMed  PubMed Central  CAS  Google Scholar 

Download references

Acknowledgements

This work was financially supported by the National Basic Research Program of China (Grant No. 2013FY111700-2), the Beijing Talents Training Program (Grant No. 2014000020060G179), the Special Fund for the Construction of Scientific and Technological Innovation Capability (Grant No. KJCX20170111) and the Young Foundation of Beijing Academy of Agriculture and Forestry Sciences (Grant No. QNJJ201703). We thank Robbie Lewis, MSc, from Liwen Bianji, Edanz Group China (http://www.liwenbianji.cn/ac), for editing the English text of a draft of this manuscript.

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Correspondence to Guanglong Hu.

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Lan, Y., Cheng, L., Huang, W. et al. The complete chloroplast genome sequence of Actinidia kolomikta from north China. Conservation Genet Resour 10, 475–477 (2018). https://doi.org/10.1007/s12686-017-0852-8

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