Isolation and characterization of SNPs in bighead carp
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Bighead carp (Aristichthys nobilis) is one of the most economically and ecologically important fish species widely stocked in lakes or cultured in ponds throughout China. The natural populations are facing the stringent challenges of germplasm resources reduction because of pollution and culture. Thus, developing sufficient molecular markers for the conservation and molecular-aided selection of bighead carp is urgent. In this study, we used SNaPshot to develop single nucleotide polymorphism (SNP) markers in bighead carp. A total of 38861 putative SNP markers were identified from the RAD sequencing of bighead carp. Among the markers, 28 SNPs were randomly selected and 25 loci exhibited significant polymorphisms in genotyping by SNaPshot. To the best of our knowledge, this is the first report about SNP marker development in bighead carp based on SNaPshot. These SNP markers can provide an effective tool for the resource conservation and genetic improvement of bighead carp.
KeywordsAristichthys nobilis Single nucleotide polymorphism (SNP) SNaPshot
This study was supported by the Special Fund for Agro-scientific Research in the Public Interest, State Agriculture Ministry of China (201303056), the National Natural Science foundation of China (31072218) and by the Shanghai University Knowledge Service Platform Project (ZF1206).
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