Characterization of the complete chloroplast genome of the Tertiary relict tree Phellodendron amurense (Sapindales: Rutaceae) using Illumina sequencing technology
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The Amur cork tree (Phellodendron amurense) is an endangered deciduous tree species with high medicinal and economic values. In this study, its complete chloroplast genome was determined using high-throughput Illumina sequencing technology. The cp genome of P. amurense is 158,442 bp in length, and contains a pair of inverted repeat (IR) regions of 26,999 bp each, separated by a large single-copy (LSC) region of 86,160 bp and a small single-copy (SSC) region of 18,284 bp. It encodes 113 gene species (including 79 PCG, 30 tRNA and 4 rRNA species) with 21 of them occurring in double copies. The nucleotide composition is asymmetric (30.5% A, 19.6% C, 18.8% G & 31.1% T) with an overall A+T content of 61.6%. Phylogenetic analysis supports the morpho-taxonomy of the order Sapindales, and indicates that P. amurense is relatively closely related to the genus Zanthoxylum (as represented by Zanthoxylum bungeanum, Zanthoxylum piperitum and Zanthoxylum schinifolium).
KeywordsAmur cork tree Phellodendron amurense Relict species High-throughput sequencing Plastid genome
The study was supported by the Special Agricultural Plan of Xi’an Science & Technology Bureau [NC1403(3)] and the Special Scientific Research Project of the Education Department of Shaanxi Province [15JK2140].
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