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Development of 42 SNP markers for the Chinese mitten crab Eriocheir sinensis based on transcriptomics

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Abstract

The Chinese mitten crab (Eriocheir sinensis) is one of the most important aquaculture species in China. To discover single nucleotide polymorphisms (SNP) markers for this species, 45 samples were used as templates for PCR validation, and 42 SNP markers were developed in this study. The results showed that the minor allele frequency ranged from 0.0111 to 0.4778, and the observed heterozygosity (Ho) and expected heterozygosity (He) varied from 0.0222 to 0.6000 and 0.1061 to 0.5461, respectively. Among these SNPs, seven loci deviated significantly from the Hardy–Weinberg equilibrium (P < 0.05). These SNPs will facilitate the assessment of genetic diversity and selective breeding programs for E. sinensis.

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References

  • Cui Z, Li X, Liu Y, Song C, Hui M, Shi G, Luo D, Li Y (2013) Transcriptome profiling analysis on whole bodies of microbial challenged Eriocheir sinensis larvae for immune gene identification and SNP development. PLoS ONE 8:e82156. doi:10.1371/journal.pone.0082156

    Article  PubMed  PubMed Central  Google Scholar 

  • He J, Wu X, Li J, Huang Q, Huang Z, Cheng Y (2014) Comparison of the culture performance and profitability of wild-caught and captive pond-reared Chinese mitten crab (Eriocheir sinensis) juveniles reared in grow-out ponds: Implications for seed selection and genetic selection programs. Aquaculture 434:48–56. doi:10.1016/j.aquaculture.2014.07.022

    Article  Google Scholar 

  • Liu Q, Liu H, Wu X, He J, Dong P, Wang Y, Cheng Y (2015) Genetic variation of wild and cultured populations of Chinese mitten crab Eriocheir sinensis from the Yangtze, Huanghe, and Liaohe River basins using microsatellite markers. Oceanol Et Limnol Sin 46:958–968. doi:10.11693/hyhz20150400129

    Google Scholar 

  • Lv J, Zhang D, Gao B, Liu P, Li J (2015) Transcriptome and MassARRAY analysis for identification of transcripts and SNPs for growth traits of the swimming crab Portunus trituberculatus. Gene 566:229–235. doi:10.1016/j.gene.2015.04.050

    Article  CAS  PubMed  Google Scholar 

  • Sambrook J, Russell D (2001) Molecular cloning: a laboratory manual; 3rd edn. Cold Spring Harbor Laboratory press. New York, pp 479–783

    Google Scholar 

  • Sellars MJ, Dierens L, McWilliam S, Little B, Murphy B, Coman GJ, Barendse W, Henshall J (2014) Comparison of microsatellite and SNP DNA markers for pedigree assignment in Black Tiger shrimp,Penaeus monodon. Aquacult Res 45:417–426. doi:10.1111/j.1365-2109.2012.03243.x

    Article  CAS  Google Scholar 

  • Sui L, Zhang F, Wang X, Bossier P, Sorgeloos P, Hänfling B (2009) Genetic diversity and population structure of the Chinese mitten crab Eriocheir sinensis in its native range. Mar Biol 156:1573–1583. doi:10.1007/s00227-009-1193-2

    Article  Google Scholar 

  • Zhang M, Nie J, Shen Y, Xu X, Dang Y, Wang R, Li J (2015) Isolation and characterization of 25 novel EST-SNP markers in grass carp (Ctenopharyngodon idella). Conserv Genet Resour 7:819–822. doi:10.1007/s12686-015-0491-x

    Article  Google Scholar 

  • Zhao H, Wu X, Long X, He J, Jiang X, Liu N, Cheng Y (2016) Nutritional composition of cultured adult male Eriocheir sinensis from Yangtze River, Yellow River and Liaohe River. J Fishery Sci China 23:1117–1129. doi:10.3724/sp.j.1118.2016.15452

    Google Scholar 

Download references

Acknowledgements

This work was supported by the Scientific and Technological Cooperation Project [Grant Number 2014DFT30270], the Innovation Fund for Small and Medium-sized Technology-based Firms [Grant Number 14C26213201214], the Ministry of Science and Technology of China and an extension project [Grant Number 2015-1-7], the Shanghai Universities Top Disciplines Project [Grant Number 2015-62-0908], and the Capacity Promotion Projects [Grant Number 16DZ2281200, 13DZ2280500].

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Authors and Affiliations

  1. Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Lingang New District, Shanghai, 201306, China

    Qizhen Xiao, Qing Liu, Pengsheng Dong, Xugan Wu & Yongxu Cheng

  2. Collaborative Innovation Center of Aquatic Animal Breeding Center Certificated by Shanghai Municipal Education Commission, Shanghai Ocean University, Lingang New District, Shanghai, 201306, China

    Qizhen Xiao, Qing Liu, Pengsheng Dong & Yongxu Cheng

Authors
  1. Qizhen Xiao
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  2. Qing Liu
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  3. Pengsheng Dong
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  4. Xugan Wu
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  5. Yongxu Cheng
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Corresponding authors

Correspondence to Xugan Wu or Yongxu Cheng.

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Xiao, Q., Liu, Q., Dong, P. et al. Development of 42 SNP markers for the Chinese mitten crab Eriocheir sinensis based on transcriptomics. Conservation Genet Resour 9, 375–377 (2017). https://doi.org/10.1007/s12686-017-0687-3

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  • DOI: https://doi.org/10.1007/s12686-017-0687-3

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