Abstract
The whole chloroplast (cp) genome sequence of Camptotheca acuminata has been characterized from Illumina pair-end sequencing. The complete cp genome was 162,382 bp in length, containing a large single copy region (LSC) of 89,493 bp and a small single copy region (SSC) of 16,540 bp, which were separated by two inverted repeat (IR) regions of 56,648 bp. The genome contained 129 genes, including 83 protein-coding genes, 38 tRNA genes and 8 ribosomal RNA genes (4 rRNA species). The most of gene species occur as a single copy, while 18 gene species occur in double copies. The overall GC content of C. acuminata cp genome is 38%, while the corresponding values of the LSC, SSC and IR regions are 36.2, 32.7 and 42.3%, respectively. Further, phylogenetic analysis suggested that C. acuminata is closely related to the species of Davidia involucrata.
References
Doyle JJ (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15
Huang DI, Cronk QCB (2015) Plann: a command-line application for annotating plastome sequences. Appl Plant Sci 3:1500026
Katoh K, Standley DM (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol 30:772–780
Kearse M, Moir R, Wilson A et al (2012) Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28:1647–1649
Li S, Zhang Z, Cain A, Wang B, Long M, Taylor J (2005) Antifungal activity of camptothecin, trifolin, and hyperoside isolated from Camptotheca acuminata. J Agric Food Chem 53(1):32–37
Lohse M, Drechsel O, Kahlau S et al (2013) OrganellarGenomeDRAW: a suite of tools for generating physical maps of plastid and mitochondrial genomes and visualizing expression data sets. Nucleic Acids Res 41:W575–W581
Maier RM, Neckermann K, Igloi GL, Kössel H (1995) Complete sequence of the maize chloroplast genome: gene content, hotspots of divergence and fine tuning of genetic information by transcript editing. J Mol Biol 251(5):614–628
Oberlies NH, Kroll DJ (2004) Camptothecin and Taxol: historic achievements in natural products research. J Nat Prod 67(2):129–135
Stamatakis A (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30:1312–1313
Yu T, Lv J, Li J, Du FK, Yin K (2016) The complete chloroplast genome of the dove tree Davidia involucrata (Nyssaceae), a relict species endemic to China. Conserv Genet Res 8(3):263–266
Zerbino DR, Birney E (2008) Velvet: algorithms for de novo short read assembly using de Bruijn graphs. Genome Res 18:821–829
Acknowledgements
This work was financially supported by the National Natural Science Foundation of China (31500502).
Author information
Authors and Affiliations
Corresponding author
Additional information
Weiwei Wang and Hang Liu have contributed equally to this work.
Electronic supplementary material
Below is the link to the electronic supplementary material.
12686_2016_662_MOESM1_ESM.pdf
Supplementary Figure S1. The phylogenetic tree based on the 11 complete chloroplast genome sequences. Accession numbers: Camptotheca acuminate KY197962, Davidia involucrate KX021372, Diplopanax stachyanthus NC_029750, Hydrangea serrata KU140669, Swida controversa KU852492, Nicotiana tabacum Z00044, Atropa belladonna NC_004561, Panax ginseng KF431956, Actinidia chinensis KP297245, pinacia oleracea AJ400848, Zea mays NC_001666. (PDF 331 KB)
Rights and permissions
About this article
Cite this article
Wang, W., Liu, H., He, Q. et al. Characterization of the complete chloroplast genome of Camptotheca acuminata . Conservation Genet Resour 9, 241–243 (2017). https://doi.org/10.1007/s12686-016-0662-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-016-0662-4