Abstract
The small abalone, Haliotis diversicolor, is an economically important shellfish species in East Asia. The wild stocks have been highly threatened due to overfishing in the past several decades. Taking into account conservation and recovery of wild resources, long preserved samples were used to analyze population structure. However, extreme fragmentation of DNA limited the use of these degraded DNA samples. In this study, a total of 96 novel single nucleotide polymorphisms (SNPs) were identified based on transcriptomes of H. diversicolor. The parallel target sequencing was then used to examine one wild population collected in 2003 from Sanya Bay, Hainan Province with degraded DNA. Nineteen loci appeared in more than 80% of individuals and were polymorphic. This study provides a valuable method for using degraded DNA and molecular markers for genetic diversity analysis of H. diversicolor.
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Acknowledgements
This work was supported by grants from National Natural Science Foundation of China (No. 31472277 and U12050091), Fundamental Research Funds for the Central Universities (No. 20720150077), Key S&T Program of Fujian Province (No. 2016NZ01010006), Earmarked Fund for Modern Agro-industry Technology Research System (No. CARS-48) and EUR-cooperative Foundation of Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources (No. FJMBIO1506).
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Ren, P., Shen, Y., Huang, Z. et al. SNP detection by parallel targeted sequencing from degraded DNA samples in Haliotis diversicolor . Conservation Genet Resour 9, 193–195 (2017). https://doi.org/10.1007/s12686-016-0648-2
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DOI: https://doi.org/10.1007/s12686-016-0648-2