Noninvasive genetic methods enable the sampling of natural populations while minimizing detrimental effects on them. However, noninvasive methods are marred by DNA extraction and amplification difficulties that can be mitigated by improved methodology. Past studies have shown that noninvasive genetic protocols are system specific and highlighted the importance of pilot studies in the establishment of genetic monitoring programs. We tested, using a factorial design experiment, the effect of different methods for the collection-preservation and extraction on the amplification of DNA from Nubian ibex (Capra nubiana) fecal samples. We found collection-preservation using paper bags and extraction with QIAamp® fast DNA stool mini kit to significantly enhance success rate compared to collection-preservation in ethanol and extraction with QIAamp® DNA mini kit. Our results will contribute to the studies of natural populations of the vulnerable C. nubiana.
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We would like to thank the regional biologists and rangers of the Israel Nature and Parks Authority for their support and assistance. We thank Ofer Ovadia and Sharon Renan for fruitful communication. This research was supported by the Israeli Nature and National Parks Protection Authority and the Israeli Ministry of Environmental Protection as part of the program for development and rehabilitation of the Dead Sea Basin. This is publication XXX of the Mitrani Department of Desert Ecology, Ben-Gurion University of the Negev.
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Goodman, I., Shahar, N., Bar-Gal, G.K. et al. Evaluation of noninvasive genetic methods for Nubian ibex. Conservation Genet Resour 9, 181–183 (2017). https://doi.org/10.1007/s12686-016-0642-8
- Fecal DNA
- DNA extraction
- Capra nubiana
- Nubian ibex