Abstract
Though used as a common model species in numerous studies, sex determination of Peromyscus has relied solely on visual methods. By combining two sex chromosome primers, LGL331/LGL335 and Jarid1, in a new multiplex amplification reaction we developed a molecular method for Peromyscus sex determination.
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References
Bakloushinskaya IY (2009) Evolution of sex determination in mammals. Biol Bull 36:167–174. doi:10.1134/s1062359009020095
Cathey JC, Bickham JW, Patton JC (1998) Introgressive hybridization and nonconcordant evolutionary history of maternal and paternal lineages in North American deer. Evol Int J org Evol 52:1224–1229. doi:10.2307/2411253
Chappel M (1988) Hemoglobin polymorphisms in deer mice (Peromyscus maniculatus): physiology of beta-globin variants and alpha-globin recombinants. Evol Int J org Evol 42:684–688. doi:10.2307/2408859
Clapcote SJ, Roder JC (2005) Simplex PCR assay for sex determination in mice. Biotechniques 38:702–706. doi:10.2144/05385BM05
Csiszar A, Labinskyy N, Zhao X et al (2007) Vascular superoxide and hydrogen peroxide production and oxidative stress resistance in two closely related rodent species with disparate longevity. Aging Cell 6:783–797. doi:10.1111/j.1474-9726.2007.00339.x
Jašarević E, Williams SA, Vandas GM et al (2013) Sex and dose-dependent effects of developmental exposure to bisphenol A on anxiety and spatial learning in deer mice (Peromyscus maniculatus bairdii) offspring. Horm Behav 63:180–189. doi:10.1016/j.yhbeh.2012.09.009
Lewis MH, Tanimura Y, Lee LW, Bodfish JW (2007) Animal models of restricted repetitive behaviour in autism. Behav Brain Res 176:66–74. doi:10.1016/j.bbr.2006.08.023.Animal
O’Neill R, Szalai G, Gibbs R et al (1998) White paper proposal for sequencing the genome of Peromyscus. National Human Genome Research Institute. https://www.genome.gov/Pages/Research/Sequencing/SeqProposals/Peromyscus.pdf. Accessed 25 March 2016
Parnell PG, Crossland JP, Beattie RM, Dewey MJ (2005) Frequent Harderian gland adenocarcinomas in inbred white-footed mice (Peromyscus leucopus). Comp Med 55:382–386
Trainor BC, Pride MC, Villalon Landeros R et al (2011) Sex differences in social interaction behavior following social defeat stress in the monogamous California mouse (Peromyscus californicus). PLoS One 6:e17405. doi:10.1371/journal.pone.0017405
Acknowledgment
Peromyscus samples were collected for this research using protocols approved by the Animal Care Committees at Trent University and the University of Guelph, following the standards and guidelines established by the Canadian Council on Animal Care. We would like to thank Claire Jardine, Andrew McAdam, Trent University’s Animal Care Facility, and the Riverview Park & Zoo for their sample donation. This work was supported by Natural Sciences and Engineering Research Council of Canada Discovery Grants to JB and PJW and by the Wildlife Research and Monitoring Section of the Ontario Ministry of Natural Resources and Forestry.
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Morris, K.Y., Bowman, J., Kerr, M. et al. Molecular sex determination of Peromyscus maniculatus and Peromyscus leucopus . Conservation Genet Resour 9, 103–105 (2017). https://doi.org/10.1007/s12686-016-0631-y
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DOI: https://doi.org/10.1007/s12686-016-0631-y