Using environmental DNA to detect an endangered crayfish Cambaroides japonicus in streams
- 585 Downloads
The freshwater crayfish, Cambaroides japonicus, is endangered in Hokkaido, Japan and inhabits burrows. Here, we applied environmental DNA (eDNA) method for evaluating the species distributions with comparing hand-capture method in the headwater streams. We detected the eDNA of C. japonicus from all sites, where we collected C. japonicus, and confirmed that eDNA can be applied to detect burrowing aquatic freshwater crayfish without disturbing their habitats.
KeywordsSpecies-specific primers Real-time PCR Lotic system Species detection
- Kawai T, Nakata K, Hamano T (2002) Temporal changes of the density in two crayfish species, the native Cambaroides japonicus (De Haan) and the alien Pacifastacus leniusculus (Dana), in natural habitats of Hokkaido, Japan. Freshw Crayfish 13:198–206Google Scholar
- R Core Team (2015) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. https://www.R-project.org/