Conservation Genetics Resources

, Volume 8, Issue 3, pp 231–234 | Cite as

Using environmental DNA to detect an endangered crayfish Cambaroides japonicus in streams

  • Kousuke Ikeda
  • Hideyuki Doi
  • Kazunori Tanaka
  • Tadashi Kawai
  • Junjiro N. Negishi
Technical Note

Abstract

The freshwater crayfish, Cambaroides japonicus, is endangered in Hokkaido, Japan and inhabits burrows. Here, we applied environmental DNA (eDNA) method for evaluating the species distributions with comparing hand-capture method in the headwater streams. We detected the eDNA of C. japonicus from all sites, where we collected C. japonicus, and confirmed that eDNA can be applied to detect burrowing aquatic freshwater crayfish without disturbing their habitats.

Keywords

Species-specific primers Real-time PCR Lotic system Species detection 

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Kousuke Ikeda
    • 1
  • Hideyuki Doi
    • 2
  • Kazunori Tanaka
    • 3
  • Tadashi Kawai
    • 4
  • Junjiro N. Negishi
    • 1
  1. 1.Graduate School of Environmental ScienceHokkaido UniversitySapporoJapan
  2. 2.Graduate School of Simulation StudiesUniversity of HyogoKobeJapan
  3. 3.Research Group of Regional Sciences, Graduate School of LettersHokkaido UniversitySapporoJapan
  4. 4.Wakkanai Fisheries Research InstituteWakkanaiJapan

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