Sex identification of the masked palm civet (Paguma larvata) using noninvasive hair samples
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The masked palm civet (Paguma larvata) is one of the most common mesocarnivores in the mountain ecosystem of Southwest China, where mesocarnivores may play critical roles due to loss of apex predators. Given their elusive nature, genetic analysis based on noninvasive survey techniques is a promising approach to determine the population dynamics and ecological functions of masked palm civets. Here we describe a method for molecular sex identification of masked palm civets. Two pairs of primers were designed to amplify fragments of zfx and sry genes, respectively. Through PCR amplification, the expected fragments, two (123 and 244 bp) in males and one (123 bp) in females, were successfully obtained in 8 sex-known hair samples. The procedures we developed here represent a reliable method for sex identification of masked palm civets.
KeywordsMasked palm civet Sex identification zfx gene sry gene Noninvasive sampling
This study was funded by The Nature Conservancy China and the Sichuan Nature Conservation Foundation. We are grateful to Yanping Lu from Beijing Zoo and the field staff of Laohegou Nature Reserve for sample collection.
- Bu HL, Hopkins JB III, Zhang D, Li S, Wang RJ, Yao M, Wang DJ (2016) An evaluation of hair-snaring devices for small-bodied carnivores in Southwest China. J Mammal 97(2):589–598Google Scholar
- Jennings AP, Veron G (2009) Family Viverridae. In: Wilson D, Mittermeier RA (eds) Handbook of the mammals of the world, Vol. 1, carnivores. Lynx edicions, Barcelona, pp 174–233Google Scholar