A novel molecular method for noninvasive sex identification of order Carnivora

Abstract

Noninvasive sampling can provide an efficient means of genetically monitoring mammals. Due to the fragmented quality of DNA derived from such samples, few methods span taxonomic groups beyond species. Here, we describe a more universal protocol for the molecular sex identification of order Carnivora. PCR amplification of a 176 bp segment of the SRY gene indicates a male, whereas no amplification indicates a female. A 245 bp segment of the ZFX gene serves as a positive control. This method is internally consistent, and multiple trials confer confidence in results. It applies to at least three families (Canidae, Felidae, and Phocidae) in both carnivore suborders (Feliformia and Caniformia), rendering this protocol the first sex identification method with amplicon sizes appropriate for noninvasive samples suitable for multiple families within order Carnivora.

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References

  1. Caragiulo A, Dougherty E, Soto S et al (2014) The complete mitochondrial genome structure of the jaguar (Panthera onca). Mitochondrial DNA 1–2

  2. González S, Mannise N, Repetto L, Maldonado JE (2015) Sex determination of three Neotropical canids by high resolution melting analysis. Conserv Genet Resour 7:1–3

    Article  Google Scholar 

  3. Kearse MM, Wilson A, Stones-Havas S et al (2012) Geneious basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28:1647–1649

    Article  PubMed  PubMed Central  Google Scholar 

  4. Murphy W, Eizirik E, Johnson W (2001) Molecular phylogenetics and the origins of placental mammals. Nature 409:614–618

    CAS  Article  PubMed  Google Scholar 

  5. Pagès M, Maudet C, Bellemain E et al (2009) A system for sex determination from degraded DNA: a useful tool for palaeogenetics and conservation genetics of ursids. Conserv Genet 10:897–907

    Article  Google Scholar 

  6. Pilgrim KL, Mckelvey KS, Riddle AE, Schwartz MK (2005) Felid sex identification based on noninvasive genetic samples. Mol Ecol Notes 5:60–61

    CAS  Article  Google Scholar 

  7. Trinca CS, Eizirik E (2012) Molecular sexing of Neotropical otter (Lontra longicaudis) noninvasive samples. Conserv Genet Resour 4:575–577

    Article  Google Scholar 

  8. Waits LP, Paetkau D (2005) Noninvasive genetic sampling tools for wildlife biologists: a review of applications and recommendations for accurate data collection. J Wildl Manage 69:1419–1433

    Article  Google Scholar 

  9. Wei K, Zhang Z, Zhang W et al (2007) PCR-CTPP: a rapid and reliable genotyping technique based on ZFX/ZFY alleles for sex identification of tiger (Panthera tigris) and four other endangered felids. Conserv Genet 9:225–228

    Article  Google Scholar 

Download references

Acknowledgments

We would like to thank the Black Pine Animal Sanctuary (Albion, IN), Cedar Cove Feline Conservatory and Education Center (Louisburg, KS), International Exotic Animal Sanctuary (Boyd, TX), Long Island Aquarium (Riverhead, NY), Octagon Wildlife Sanctuary (Punta Gorda, FL), and Global Felid Genetics Program (New York, NY) for providing control samples. Funding was provided by the Sackler Institute for Comparative Genomics at the American Museum of Natural History and the Department of Ecology, Evolution, and Environmental Biology at Columbia University.

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Correspondence to Alexandra DeCandia.

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DeCandia, A., Gaughran, S., Caragiulo, A. et al. A novel molecular method for noninvasive sex identification of order Carnivora. Conservation Genet Resour 8, 119–121 (2016). https://doi.org/10.1007/s12686-016-0525-z

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Keywords

  • Noninvasive DNA sampling
  • Sex assignment
  • Carnivora
  • SRY
  • Canidae
  • Felidae