Invasive alien species are one of the leading causes of extinctions worldwide. Preventing their establishment, eradicating or containing their spread relies on low-density detection. Environmental DNA (eDNA) has been shown to have superior detection sensitivity compared to traditional methods, permitting detection at lower densities. Here, we develop a species-specific assay to detect Perca fluviatilis (redfin perch), an invasive freshwater fish present in Australia. We show that the assay is highly sensitive and highly specific to detect the species in Australian freshwater environments and demonstrate the utility of the assay for detecting the species from environmental water samples. This assay will prove useful for low-density detection of P. fluviatilis to assist in the management of this invader, with conservation benefits for the native species it threatens.
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Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410. doi:10.1016/S0022-2836(05)80360-2
Benson DA, Karsch-Mizrachi I, Lipman DJ, Ostell J, Sayers EW (2009) GenBank. Nucleic Acids Res 37:D26–31
Betancur-R R et al. (2013) The tree of life and a new classification of bony fishes. PLoS Curr 5. doi:10.1371/currents.tol.53ba26640df0ccaee75bb165c8c26288
Bomford M, O’Brien P (1995) Eradication or control for vertebrate pests? Wildl Soc Bull 23:249–255
Clavero M, García-Berthou E (2005) Invasive species are a leading cause of animal extinctions. Trends Ecol Evol 20:110
Dejean T, Valentini A, Miquel C, Taberlet P, Bellemain E, Miaud C (2012) Improved detection of an alien invasive species through environmental DNA barcoding: the example of the American bullfrog Lithobates catesbeianus. J Appl Ecol 49:953–959. doi:10.1111/j.1365-2664.2012.02171.x
Fuller S, Baverstock P, King D (1998) Biogeographic origins of goannas (Varanidae): a molecular perspective. Mol Phylogenet Evol 9:294–307. doi:10.1006/mpev.1997.0476
Furlan EM, Gleeson DM, Hardy CM, Duncan RP (2015) A framework for estimating the sensitivity of eDNA surveys. Mol Ecol Resour. doi:10.1111/1755-0998.12483
Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis. Nucleic Acids Symp Ser 41:95–98
Hardy CM, Adams M, Jerry DR, Court LN, Morgan MJ, Hartley DM (2011) DNA barcoding to support conservation: species identification, genetic structure and biogeography of fishes in the Murray–Darling River Basin, Australia. Mar Freshw Res 62:887–901. doi:10.1071/mf11027
Janosik AM, Johnston CE (2015) Environmental DNA as an effective tool for detection of imperiled fishes. Environ Biol Fish 98:1889–1893. doi:10.1007/s10641-015-0405-5
Jerde CL, Mahon AR, Chadderton WL, Lodge DM (2011) “Sight-unseen” detection of rare aquatic species using environmental DNA. Conserv Lett 4:150–157. doi:10.1111/j.1755-263X.2010.00158.x
Lintermans M (2001) Fish in the Upper Murrumbidgee Catchment. A review of Current knowledge. Wildlife Research & Monitoring, Canberra
Lintermans M (2007) Fishes of the Murray–Darling Basin: an introductory guide. Murray-Darling Basin Authority, Canberra
McLeod R (2004) Counting the cost: impact of invasive animals in Australia. Cooperative Research Centre for Pest Animal Control, Canberra
Rowe D, Moore A, Giorgetti A, Maclean C, Grace P, Wadhwa S, Cooke J (2008) Review of the impacts of gambusia, redfin perch, tench, roach, yellowfin goby and streaked goby in Australia. Australian Government Department of the Environment, Water, Heritage and the Arts, ACT
Sigsgaard EE, Carl H, Møller PR, Thomsen PF (2015) Monitoring the near-extinct European weather loach in Denmark based on environmental DNA from water samples. Biol Conserv 183:46–52. doi:10.1016/j.biocon.2014.11.040
Simberloff D et al (2013) Impacts of biological invasions: what’s what and the way forward. Trends Ecol Evol 28:58–66. doi:10.1016/j.tree.2012.07.013
Smart AS, Tingley R, Weeks AR, van Rooyen AR, McCarthy MA (2015) Environmental DNA sampling is more sensitive than a traditional survey technique for detecting an aquatic invader. Ecol Appl 25:1944–1952. doi:10.1890/14-1751.1
Wager R, Jackson PD (1993) The action plan for Australian freshwater fishes. Australian Nature Conservation Agency, Canberra
We would like to thank the Invasive Animals Cooperative Research Centre (Project 1.W.2) for funding to conduct this research. We are grateful to C. M. Hardy, M. Lintermans and P. Unmack for advice and access to DNA samples, and to three anonymous reviewers for comments on an earlier version of this manuscript. Animal collections and holdings were carried out under ACT Government TAMS Licence to Take, Licence number LT2013661 and LT2013699 and University of Canberra CEAE 13-05.
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Furlan, E.M., Gleeson, D. Environmental DNA detection of redfin perch, Perca fluviatilis . Conservation Genet Resour 8, 115–118 (2016). https://doi.org/10.1007/s12686-016-0523-1
- Environmental DNA
- Redfin perch
- Perca fluviatilis