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SNP discovery from liver transcriptome in the fish Piaractus mesopotamicus

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Abstract

Pacu (Piaractus mesopotamicus) is a Neotropical freshwater fish threatened by overfishing, and one of the species of highest commercial value for aquaculture. Genetic variability analysis through molecular markers is an essential technique to genetic management of the wild and cultivated stocks. The main objective of this study was to identify and validate gene-associated SNPs of the liver transcriptome of pacu. Through genetic analysis in one natural population (Paraná River), 32 polymorphic SNPs were successfully genotyped and validated, some of them related to immune system genes. The observed and expected heterozygosity ranged from 0.059 to 0.706 and 0.058 to 0.507, respectively. All loci were in Hardy–Weinberg equilibrium (P > 0.05). Our results showed useful genomic resources for pacu, with applicability in conservation purposes and aquaculture industry by using SNPs markers.

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Acknowledgments

This work was supported by grants from Pró-Reitoria de Pesquisa da UNESP (Prope 07/2015—DTH), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq 446779/2014-8 and 305916/2015-7—DTH; and 130262/2014-5 VAMF), and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP 2014/03772-7—DTH and 2014/12412-4—VAMF).

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Authors and Affiliations

  1. Centro de Aquicultura da Unesp, Universidade Estadual Paulista, Jaboticabal, SP, Brazil

    Vito Antonio Mastrochirico-Filho, Milene Elissa Hata, Lucas Seiti Sato, Paulo Henrique Jorge & Diogo Teruo Hashimoto

  2. Departamento de Morfologia, IBB, Universidade Estadual Paulista, Botucatu, SP, Brazil

    Fausto Foresti

  3. Departamento de Genética, Facultad de Veterinaria, Universidad de Santiago de Compostela, Lugo, Spain

    Manuel Vera Rodriguez & Paulino Martínez

  4. Departamento de Ciências Biológicas, FC, Universidade Estadual Paulista, Bauru, SP, Brazil

    Fábio Porto-Foresti

Authors
  1. Vito Antonio Mastrochirico-Filho
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  2. Milene Elissa Hata
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  3. Lucas Seiti Sato
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  4. Paulo Henrique Jorge
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  5. Fausto Foresti
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  6. Manuel Vera Rodriguez
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  7. Paulino Martínez
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  8. Fábio Porto-Foresti
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  9. Diogo Teruo Hashimoto
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Corresponding author

Correspondence to Diogo Teruo Hashimoto.

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Mastrochirico-Filho, V.A., Hata, M.E., Sato, L.S. et al. SNP discovery from liver transcriptome in the fish Piaractus mesopotamicus . Conservation Genet Resour 8, 109–114 (2016). https://doi.org/10.1007/s12686-016-0521-3

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  • DOI: https://doi.org/10.1007/s12686-016-0521-3

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