Abstract
The F1 hybrids of female channel catfish × male blue catfish contain many desirable traits for commercial production. But hybrid catfish cannot always be easily distinguished from channel catfish. It is also difficult to identify fingerlings and fillets of blue catfish, channel catfish and F1 hybrids. The present research showed that a trinucleotide microsatellite in the myostatin (MSTN) gene had two species-specific alleles allowing the identification of blue catfish, channel catfish, and the F1 hybrids. This molecular marker will be used to identify fingerlings and products among blue catfish, channel catfish and F1 hybrids.
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References
Bosworth BG, Wolters WR, Wise DJ, Li MH (1998) Growth, feed conversion, fillet proximate composition, and resistance to Edwardsiella ictaluri of channel catfish, Ictalurus punctatus (Rafinesque), blue catfish, Ictalurus furcatus (Lesueur), and their reciprocal F1 hybrids fed 25 % and 45 % protein diets. Aquac Res 29:251–257
Bosworth BG, Wise DJ, Terhune JS, Wolters WR (2003) Family and genetic group effects for resistance to proliferative gill disease in channel catfish, blue catfish, and channel catfish × blue catfish backcross hybrids. Aquac Res 34:569–573
Chen XH, Liu PP, Wang MH, Chen YM, Zhong LQ, Pan JL (2013) SNP sites of MSTN gene and its association with body weight in yellow catfish. Oceanol ET Limnol Sin 44:1566–1569
Dunham RA, Brummett RE (1999) Response of two generations of selection to increased body weight in channel catfish, Ictalurus punctatus, compared to hybridization with blue catfish, I. furcatus, males. J Appl Aquac 9:37–45
Dunham RA, Smitherman RO, Webber C (1983) Relative tolerance of channel × blue hybrid and channel catfish to low oxygen concentrations. Progress Fish Cultur 45:55–56
Dunham RA, Smitherman RO, Goodman RK (1987) Comparison of mass selection, crossbreeding, and hybridization for improving body weight in channel catfish. Progress Fish Cultur 49:293–296
Elisabeth N, Bruria F (2011) Myostatin-2 gene structure and polymorphism of the promoter and first intron in the marine fish sparus aurata: evidence for DNA duplications and/or translocations. BMC Genet 12:22
Fishery Bureau MOA (2014) China fishery statistical yearbook. China Agricultural Press, Beijing
Giudice JJ (1966) Growth of a blue × channel catfish hybrid as compared to its parent species. Progress Fish Cultur 26:142–145
Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
Hickford JG, Forrest RH, Zhou H, Fang Q, Han J, Frampton CM, Horrell AL (2010) Polymorphisms in the ovine myostatin gene (MSTN) and their association with growth and carcass traits in New Zealand Romney sheep. Anim Genet 41:64–72
Levinson G, Gutman GA (1987) High frequency of short frameshifts in poly-CA/GT tandem repeats borne by bacteriophage M13 in Escherichia coli K-12. Nucleic Acids Res 15:5323–5338
Li MH, Robinson EH, Manning BB, Yant DR, Chatakondi NG, Bosworth BG, Wolters WR (2004) Comparison of the channel catfish, Ictalurus punctatus (NWAC103 strain) and the channel × blue catfish, I. punctatus × I. furcatus, F1 hybrid for growth, feed efficiency, processing yield, and body composition. J Appl Aquac 15:63–71
McPherron AC, Lawler AM, Lee SJ (1997) Regulation of skeletal muscle mass in mice by a new TGF-β superfamily member. Nature 387:83–90
Peñaloza C, Hamilton A, Guy DR, Bishop SC, Houston RD (2013) A SNP in the 5′ flanking region of the myostatin-1b gene is associated with harvest traits in Atlantic salmon (salmo salar). BMC Genet 14:112
Perrier C, Grandjean F, Gentil JL, Cherbonnel C, Evanno G (2011) A species-specific microsatellite marker to discriminate European Atlantic salmon, brown trout, and their hybrids. Conserv Genet Resour 3:131–133
Sellick GS, Pitchford WS, Morris CA, Cullen NG, Crawford AM, Raadsma HW, Bottema CD (2007) Effect of myostatin F94L on carcass yield in cattle. Anim Genet 38:440–446
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882
USDA (2013) Catfish production. National Agricultural Statistics Service, Agricultural Statistics Board, United States Department of Agriculture, Washington, DC
Wolters WR, Wise DJ, Klesius PH (1996) Survival and antibody response of channel catfish, blue catfish, and channel catfish female × blue catfish male hybrids after exposure to Edwardsiella ictaluri. J Aquat Anim Health 9:249–254
Zhu J, Zhong LQ, Zhang CF, Liu H, Li B (2011) Sequence variation and secondary structure analysis of the first ribosomal internal transcribed spacer (ITS-1) between Cyprinus carpio carpio and C. carpio haematopterus. Biochem Genet 49:20–24
Zhu YY, Liang HW, Li Z, Luo XZ, Li L, Zhang ZW, Zou GW (2012) Polymorphism of MSTN gene and its association with growth traits in yellow catfish (Pelteobagruse fulvidraco). Hereditas (Beijing) 34(1):72–78
Acknowledgments
This study was supported by Grants from the National Key Technology R&D Program of China (2012BAD26B03), the Fund for Independent Innovation on Agriculture Science and Technology of Jiangsu Province [CX(12)2040] and [CX(15)1013], Science and Technology Support Program of Jiangsu Province (BE2013445), the project of human resources and social security of Jiangsu Province (2014-NY-008) and the project for aquaculture in Jiangsu Province (Y2014-25).
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Zhong, L., Chen, X., Wang, M. et al. A species-specific microsatellite marker to discriminate blue catfish, channel catfish and F1 hybrids. Conservation Genet Resour 7, 827–829 (2015). https://doi.org/10.1007/s12686-015-0502-y
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DOI: https://doi.org/10.1007/s12686-015-0502-y