Drinking water as a source of environmental DNA for the detection of terrestrial wildlife species
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Use of environmental DNA for wildlife species detection is a field of research that has seen rapid growth in recent years, however, the majority of research to date has been focused on aquatic species. Here, we propose and test a novel source for the detection of terrestrial species with environmental DNA: drinking water from watering holes and wildlife water developments. We hypothesized that when terrestrial animals drink from a water source, DNA from saliva and buccal cells is shed and can be isolated for species identification. We tested this hypothesis in a pilot study by filtering drinking water supplied to coyotes (Canis latrans) at a captive coyote research facility. DNA was successfully extracted from filters, amplified by the polymerase chain reaction, and sequenced, and sequences were positively identified as belonging to coyotes. We believe this environmental DNA based approach holds great promise for the detection of terrestrial species of conservation concern.
KeywordsCanis latrans Coyote eDNA
We thank Eric M. Gese for the idea to test eDNA techniques on carnivore drinking water. We also thank Julie K. Young and Stacey Brummer from the NWRC-Predator Research Facility, and John Olson and Charles Hawkins for their support. This work was funded by NASA Biodiversity and Ecological Forecasting program Grant NNX14AC40G.
- Larsen RT, Bissonette JA, Flinders JT, Whiting JC (2012) Framework for understanding the influences of wildlife water developments in the western United States. Calif Fish Game 98:148–163Google Scholar
- Piaggio AJ, Engeman RM, Hopken MW, Humphrey JS, Keacher KL, Bruce WE, Avery ML (2014) Detecting an elusive invasive species: a diagnostic PCR to detect Burmese python in Florida waters and an assessment of persistence of environmental DNA. Mol Ecol Resour 14:374–380. doi: 10.1111/1755-0998.12180 CrossRefPubMedGoogle Scholar
- Waits LP, Paetkau D (2005) Noninvasive genetic sampling tools for wildlife biologists: a review of applications and recommendations for accurate data collection. J Wildl Manag 69:1419–1433. doi: 10.2193/0022-541x(2005)69[1419:ngstfw]2.0.co;2
- Williams CL, Blejwas K, Johnston JJ, Jaeger MM (2003) A coyote in sheep’s clothing: predator identification from saliva. Wildl Soc Bull 31:926–932Google Scholar