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Development of 20 TaqMan assays differentiating the endangered shortnose and Lost River suckers

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Abstract

Accurate species identification is vital to conservation and management of species at risk. Species identification is challenging when taxa express similar phenotypic characters and form hybrids, for example the endangered shortnose sucker (Chasmistes brevirostris) and Lost River sucker (Deltistes luxatus). Here, we developed 20 Taqman assays that differentiate these species (19 nuclear DNA and one mitochondrial DNA). Assays were evaluated in 160 young-of-the-year identified to species using meristic counts. Alleles were not fixed between species, but species were highly differentiated (F ST  = 0.753, P < 0.001). The assays developed herein will be a valuable tool for resource managers.

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Acknowledgments

Samples were collected by biologists at Western Fisheries Research Center, Klamath Falls Field Station and U.S. Bureau of Reclamation, Klamath Area Office. Summer Burdick, Dan Drinan, Lisa Seeb, Jim Seeb, and Mike Miller provided advice and assistance. Mike Miller provided P1 RAD adapters. Funding was provided by the Bureau of Reclamation through Interagency Agreement R09PG20056 and by U.S. Geological Survey. Any use of trade, firm or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government.

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Authors and Affiliations

  1. Western Fisheries Research Center, U.S. Geological Survey, 6505 NE 65th Street, Seattle, WA, 98115, USA

    M. S. Hoy & C. O. Ostberg

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  1. M. S. Hoy
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  2. C. O. Ostberg
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Correspondence to C. O. Ostberg.

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Hoy, M.S., Ostberg, C.O. Development of 20 TaqMan assays differentiating the endangered shortnose and Lost River suckers. Conservation Genet Resour 7, 673–676 (2015). https://doi.org/10.1007/s12686-015-0474-y

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  • DOI: https://doi.org/10.1007/s12686-015-0474-y

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