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Development of ten polymorphic microsatellite markers for Scabiosa canescens Waldst. & Kit. (Dipsacaceae)

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Abstract

We developed ten polymorphic microsatellite markers for the herbaceous species Scabiosa canescens Waldst. & Kit. The species is currently not assessed under the IUCN Red List but has been declining historically due to intensification in agriculture and is recently declining due to habitat loss caused by agricultural abandonment of steppe-like dry grasslands. The microsatellite loci were tested for polymorphism on DNA templates extracted from 26 specimens collected across the species’ range in Germany. Across the ten loci, two to eight alleles were detected with an observed and expected heterozygosity ranging from 0.192 to 0.769 and 0.265 to 0.768, respectively. These newly developed markers provide a valuable tool for population genetic investigations required to design effective conservation measures.

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References

  • Andersson S, Waldmann P (2002) Inbreeding depression in a rare plant, Scabiosa canescens (Dipsacaceae). Hereditas 136:207–211

    Article  PubMed  Google Scholar 

  • Korneck D, Schnittler M, Vollmer I (1996) Rote Liste der Farn und Blüten pflanzen (Peridophyta et Spermatophyta) Deutschlands. In: Ludwig G, Schnittler M (eds) Rote Liste gefährdeter Pflanzen Deutschlands. Bundesamt für Naturschutz, Bonn-Bad Godesberg, pp 21–187

    Google Scholar 

  • Marshall TC, Slate J, Kruuk LEB, Pemberton JM (1998) Statistical confidence for likelihood-based paternity inference in natural populations. Mol Ecol 7:639–655

    Article  CAS  PubMed  Google Scholar 

  • Meglecz E, Costedoat C, Dubut V, Gilles A, Malausa T, Pech N, Martin J-F (2010) QDD: a user-friendly program to select microsatellite markers and design primers from large sequencing projects. Bioinformatics 26:403–404

    Article  CAS  PubMed  Google Scholar 

  • Rousset F (2008) GENEPOP’007: a complete re-implementation of the GENEPOP software for Windows and Linux. Mol Ecol Res 8:103–106

    Article  Google Scholar 

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Acknowledgments

The work was financed by the Federal Agency for Nature Conservation (BfN) Germany under the grant number FKZ 3512-86-0400. Illumina sequencing was conducted at the Berlin Center for Genomics in Biodiversity Research (BeGenDiv). This is Publication No. 15 of the BeGenDiv.

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Correspondence to Sascha A. Ismail.

Appendix

Appendix

See Table 2.

Table 2 Locations and voucher information of sampled populations

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Ismail, S.A., Duwe, V.K., Fleischer-Notter, H. et al. Development of ten polymorphic microsatellite markers for Scabiosa canescens Waldst. & Kit. (Dipsacaceae). Conservation Genet Resour 7, 725–728 (2015). https://doi.org/10.1007/s12686-015-0446-2

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  • DOI: https://doi.org/10.1007/s12686-015-0446-2

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