Microsatellite markers were developed for the coral reef fish Chaetodon vagabundus using shotgun pyrosequencing. As threats to coral reefs intensify, information on larval connectivity is of increasing value for efficient conservation planning. Here, 11 novel microsatellites were characterized for 192 individuals from Papua New Guinea. The number of alleles per locus ranged from 7 to 32, while observed and expected heterozygosity values varied from 0.214 to 0.903. These markers will be used to study population structure and larval connectivity of this iconic coral reef fish in coral reefs across the Indo-Pacific.
This is a preview of subscription content,to check access.
Access this article
Almany GR, Berumen ML, Thorrold SR et al (2007) Local replenishment of coral reef fish populations in a marine reserve. Science 316:742–744. doi:10.1126/science.1140597
Almany GR, De Arruda MP, Arthofer W et al (2009) Permanent genetic resources added to molecular ecology resources database 1(May) 2009–31, July 2009. Mol Ecol Resour 9:1460–1466. doi:10.1111/j.1755-0998.2009.02759.x
Berumen ML, Almany GR, Planes S et al (2012) Persistence of self-recruitment and patterns of larval connectivity in a marine protected area network. Ecol Evol 2:444–452. doi:10.1002/ece3.208
De’ath G, Fabricius KE, Sweatman H, Puotinen M (2012) The 27-year decline of coral cover on the Great Barrier Reef and its causes. Proc Natl Acad Sci USA 109:17995–17999. doi:10.1073/pnas.1208909109
Green AL, Maypa AP, Almany GR, et al. (2014) Larval dispersal and movement patterns of coral reef fishes, and implications for marine reserve network design. Biol Rev. doi:10.1111/brv.12155
We would like to thank the Bioscience Core Lab of the King Abdullah University of Science and Technology (KAUST) for technical assistance. For general logistic and fieldwork assistance, we thank GP Jones, SR Thorrold, S Planes, HB Harrison, MA Priest, M Srinivasan, and N Tolou. Funding was provided in part by KAUST baseline research funds (to MLB), a KAUST Special Partnership Collaborative Fellowship (to MLB, SR Thorrold, and PS-A), and the Australian Research Council (ARC) (Award FT0990835 to GR Almany) as well as ARC support to GP Jones.
Electronic supplementary material
Below is the link to the electronic supplementary material.
About this article
Cite this article
Saenz-Agudelo, P., Almany, G.R., Mansour, H. et al. Characterization of 11 novel microsatellite markers for the vagabond butterflyfish, Chaetodon vagabundus . Conservation Genet Resour 7, 713–714 (2015). https://doi.org/10.1007/s12686-015-0440-8