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Development and characterization of a multiplex microsatellite panel for the mud carp (Cirrhinus molitorella)

  • Microsatellite Letters
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Abstract

A multiplex PCR system that consisted of 12 pairs of novel primers based on the transcriptome of the mud carp was developed and characterized for genetic polymorphism analysis in 64 wild individuals in our laboratory. The microsatellite loci exhibited a range of alleles of 3 < K < 7 with an average of 4.25. The observed heterozygosity ranged from 0.375 to 0.7656 with an average of 0.6198. The expected heterozygosity ranged from 0.3863 to 0.7714 with an average of 0.6322. These novel microsatellite markers will be valuable for population genetic analysis and sustainable fishery management and conservation of genetic resources for the mud carp and related species.

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Acknowledgments

This work was supported by the special public welfare industry (agriculture) research (200903045).

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Authors and Affiliations

  1. Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation of Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 510380, Guangzhou, Guangdong, China

    Yakun Wang, Jian Zhao, Wei Li, Xincheng Zhang & Xinping Zhu

  2. College of Fisheries and Life Science, Shanghai Ocean University, 201306, Shanghai, China

    Yakun Wang & Jian Zhao

Authors
  1. Yakun Wang
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  2. Jian Zhao
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  3. Wei Li
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  4. Xincheng Zhang
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  5. Xinping Zhu
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Corresponding author

Correspondence to Xinping Zhu.

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Wang, Y., Zhao, J., Li, W. et al. Development and characterization of a multiplex microsatellite panel for the mud carp (Cirrhinus molitorella). Conservation Genet Resour 7, 575–577 (2015). https://doi.org/10.1007/s12686-015-0431-9

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  • DOI: https://doi.org/10.1007/s12686-015-0431-9

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