Skip to main content

Development of 18 novel polymorphic microsatellites from Coccinella septempunctata and cross-species amplification in Coccinellidae species

Abstract

The seven spot ladybird (Coccinella septempunctata), a widely spread coccinellid in the Palearctic region, introduced in USA where it is recently identified as invasive, is used as biological control agent. Its taxonomic status, with peripheral populations phenotypically distinct, is not well defined. Understanding its population genetic structure is crucial to investigate its evolutionary history, local adaptation in peripheral populations, dispersal and invasion dynamics. In this context, we isolated and characterised, from microsatellite enrichment and next-generation sequencing, 18 polymorphic microsatellite markers. Genetic variation was assessed from four geographically distant populations. The number of alleles ranges from two to 15 per locus and per population and the observed and expected heterozygosities are similar whatever the population (0.53 and 0.640, respectively). High successful cross-amplification in additional 21 species, belonging to five coccinellid subfamilies, suggests their broad application.

This is a preview of subscription content, access via your institution.

References

  • Kajita Y, O’Neill EM, Zheng Y, Obrycki J, Weisrock D (2012) A population genetic signature of human releases in an invasive ladybeetle. Mol Ecol 21:5473–5483

    Article  PubMed  Google Scholar 

  • Kovář I (1977) A new species of the genus Coccinella (Coleoptera) from North Africa. Acta Entomol Musei Natl Pragae 39:231–235

    Google Scholar 

  • Loiseau A, Malausa T, Lombaert F, Martin JF, Estoup A (2009) Isolation and characterization of microsatellites in the harlequin ladybird, Harmonia axyridis (Coleoptera, Coccinellidae), and cross/species amplification within the family Coccinellidae. Mol Ecol Resour 9(3):934–937

    Article  CAS  PubMed  Google Scholar 

  • Malausa T, Gilles A, Meglécz E et al (2011) High-throughput microsatellite isolation through 454 GS/FLX Titanium pyrosequencing of enriched DNA libraries. Mol Ecol Resour 11:638–644

    Article  CAS  PubMed  Google Scholar 

  • Marin J, Crouau-Roy B, Hemptinne JL, Lecompte E, Magro A (2010) Coccinella septempunctata (Coleoptera, Coccinellidae): a species complex? Zool Scr 39(6):591–602

    Article  Google Scholar 

Download references

Acknowledgments

We thank A. Magro and J.-L. Hemptine for their help in obtaining samples and in providing Coccinelidae species. This work has been done in the lab EDB, part of the “Laboratoires d’Excellence” (LABEX) entitled TULIP (ANR-10-LABX-41; ANR-11-IDEX-0002-02) and CEBA (ANR-10-LABX-25-01; ANR-11-IDEX-0002-02).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Brigitte Crouau-Roy.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOC 93 kb)

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Bouanani, M.A., Magné, F., Lecompte, É. et al. Development of 18 novel polymorphic microsatellites from Coccinella septempunctata and cross-species amplification in Coccinellidae species. Conservation Genet Resour 7, 445–449 (2015). https://doi.org/10.1007/s12686-014-0392-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12686-014-0392-4

Keywords

  • Ladybirds
  • Invasive species
  • Microsatellites
  • Population genetics
  • Cross-amplification