Conservation Genetics Resources

, Volume 7, Issue 2, pp 411–413 | Cite as

Development and characterization of microsatellite markers for the toadfish Aphos porosus

  • Francisco Silva
  • Eduardo Hernández-Miranda
  • Antonio BranteEmail author
Microsatellite Letters


The toadfish Aphos porosus is a coastal benthic-demersal fish from the Southeastern Pacific coast. This species exhibits parental care and low dispersal potential, and it is subjected to bycatch by artisanal and industrial fisheries, which make A. porosus a susceptible species in conservation terms. In this work we present 18 polymorphic microsatellite loci for this species. Allelic richness varied between 2 and 8 alleles and observed heterocigocity ranged between 0.026 and 0.737. Fourteen of the 18 loci were unlinked, polymorphic and in Hardy–Weinberg equilibrium. The non-exclusion probability over all loci was very low (0.0013). These loci show potential for population genetic analyses, for studying reproductive strategies and supporting conservation policies for this species.


Benthic fish Batrachoididae Southeastern Pacific coast 454 sequencing 



We thank to Sandra Ferrada and Santiago Miranda for their assistance on this work. This study was funded by FONDECYT grants 1130868 to AB and 11100334 to EH-M. EH-M received additional funding from INCAR (FONDAP 15110027).

Supplementary material

12686_2014_383_MOESM1_ESM.doc (64 kb)
Supplementary material 1 (DOC 64 kb)


  1. Chevreux B, Wetter T, Suhai S (1999) Genome sequence assembly using trace signals and additional sequence information. Compt Sci Biol Proc Ger Conf Bioinformatics 99:45–56Google Scholar
  2. Faircloth BC (2008) MSATCOMMANDER: detection of microsatellite repeat arrays and automated, locus-specific primer design. Mol Ecol Res 8:92–94CrossRefGoogle Scholar
  3. Hernández-Miranda E, Quiñones RA, Aedo G, Díaz-Cabrera E, Cisterna J (2012) The impact of a strong natural hypoxic event on the toadfish Aphos porosus in Coliumo Bay, south-central Chile. Rev Biol Mar Ocean 47:475–487CrossRefGoogle Scholar
  4. Huang XQ, Madan A (1999) CAP3: a DNA sequence assembly program. Genome Res 9:868–877CrossRefPubMedCentralPubMedGoogle Scholar
  5. Ojeda FP, Labra FA, Muñoz AA (2000) Biogeographic patterns of Chilean littoral fishes. Rev Chil Hist Nat 73:625–641CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Francisco Silva
    • 1
  • Eduardo Hernández-Miranda
    • 2
    • 3
  • Antonio Brante
    • 1
    Email author
  1. 1.Departamento de Ecología, Facultad de CienciasUniversidad Católica de la Santísima ConcepciónConcepciónChile
  2. 2.Programa de Investigación Marina de Excelencia (PIMEX), Facultad de Ciencias Naturales y OceanográficasUniversidad de ConcepciónConcepciónChile
  3. 3.Interdisciplinary Center for Aquaculture Research (INCAR), Casilla160-CUniversidad de ConcepciónConcepciónChile

Personalised recommendations