Microsatellites of the self-compatible nemertean Prosorhochmus americanus (Hoplonemertea)

Abstract

Thousands of microsatellite loci were identified in the nemertean Prosorhochmus americanus through enrichment-isolation and next-generation sequencing protocols. We tested 48 loci for amplification and subsequently genotyped 17 loci in 30 individuals from three North American populations. Most loci were polymorphic within and across populations; six were monomorphic. The number of alleles per locus ranged from 1 to 9 (mean 4.35, SD 2.37). Cross-species amplification in four nemertean species revealed extended marker utility. These loci will enable investigations of colonization potential and natural history in this selfing, putatively introduced species.

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Acknowledgments

Funding was provided by the Virginia Academy of Science and the VCU Department of Biology. SAC was supported by the NSF GRFP under Grant No. DGE-1147383.

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Correspondence to S. A. Caplins.

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Caplins, S.A., Friedline, C.J., Brown, B.L. et al. Microsatellites of the self-compatible nemertean Prosorhochmus americanus (Hoplonemertea). Conservation Genet Resour 7, 399–401 (2015). https://doi.org/10.1007/s12686-014-0380-8

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Keywords

  • Self-fertilization
  • Nemertean
  • Colonization