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Conservation Genetics Resources

, Volume 7, Issue 2, pp 399–401 | Cite as

Microsatellites of the self-compatible nemertean Prosorhochmus americanus (Hoplonemertea)

  • S. A. Caplins
  • C. J. Friedline
  • B. L. Brown
  • J. M. Turbeville
Microsatellite Letters

Abstract

Thousands of microsatellite loci were identified in the nemertean Prosorhochmus americanus through enrichment-isolation and next-generation sequencing protocols. We tested 48 loci for amplification and subsequently genotyped 17 loci in 30 individuals from three North American populations. Most loci were polymorphic within and across populations; six were monomorphic. The number of alleles per locus ranged from 1 to 9 (mean 4.35, SD 2.37). Cross-species amplification in four nemertean species revealed extended marker utility. These loci will enable investigations of colonization potential and natural history in this selfing, putatively introduced species.

Keywords

Self-fertilization Nemertean Colonization 

Notes

Acknowledgments

Funding was provided by the Virginia Academy of Science and the VCU Department of Biology. SAC was supported by the NSF GRFP under Grant No. DGE-1147383.

Supplementary material

12686_2014_380_MOESM1_ESM.txt (3 kb)
Supplementary material 1 (TXT 3 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • S. A. Caplins
    • 1
  • C. J. Friedline
    • 2
  • B. L. Brown
    • 2
  • J. M. Turbeville
    • 2
  1. 1.University of California DavisDavisUSA
  2. 2.Virginia Commonwealth UniversityRichmondUSA

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