Microsatellites of the self-compatible nemertean Prosorhochmus americanus (Hoplonemertea)
Thousands of microsatellite loci were identified in the nemertean Prosorhochmus americanus through enrichment-isolation and next-generation sequencing protocols. We tested 48 loci for amplification and subsequently genotyped 17 loci in 30 individuals from three North American populations. Most loci were polymorphic within and across populations; six were monomorphic. The number of alleles per locus ranged from 1 to 9 (mean 4.35, SD 2.37). Cross-species amplification in four nemertean species revealed extended marker utility. These loci will enable investigations of colonization potential and natural history in this selfing, putatively introduced species.
KeywordsSelf-fertilization Nemertean Colonization
Funding was provided by the Virginia Academy of Science and the VCU Department of Biology. SAC was supported by the NSF GRFP under Grant No. DGE-1147383.
- Turbeville JM, Caplins SA (2010) First record for Prosorhochmus americanus (Nemertea Hoplonemertea) in Virginia with notes on its natural history and morphology. Banisteria 35:61–65Google Scholar