Conservation Genetics Resources

, Volume 7, Issue 2, pp 399–401

Microsatellites of the self-compatible nemertean Prosorhochmus americanus (Hoplonemertea)

  • S. A. Caplins
  • C. J. Friedline
  • B. L. Brown
  • J. M. Turbeville
Microsatellite Letters

DOI: 10.1007/s12686-014-0380-8

Cite this article as:
Caplins, S.A., Friedline, C.J., Brown, B.L. et al. Conservation Genet Resour (2015) 7: 399. doi:10.1007/s12686-014-0380-8
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Abstract

Thousands of microsatellite loci were identified in the nemertean Prosorhochmus americanus through enrichment-isolation and next-generation sequencing protocols. We tested 48 loci for amplification and subsequently genotyped 17 loci in 30 individuals from three North American populations. Most loci were polymorphic within and across populations; six were monomorphic. The number of alleles per locus ranged from 1 to 9 (mean 4.35, SD 2.37). Cross-species amplification in four nemertean species revealed extended marker utility. These loci will enable investigations of colonization potential and natural history in this selfing, putatively introduced species.

Keywords

Self-fertilization Nemertean Colonization 

Supplementary material

12686_2014_380_MOESM1_ESM.txt (3 kb)
Supplementary material 1 (TXT 3 kb)

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • S. A. Caplins
    • 1
  • C. J. Friedline
    • 2
  • B. L. Brown
    • 2
  • J. M. Turbeville
    • 2
  1. 1.University of California DavisDavisUSA
  2. 2.Virginia Commonwealth UniversityRichmondUSA

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