Conservation Genetics Resources

, Volume 7, Issue 2, pp 399–401 | Cite as

Microsatellites of the self-compatible nemertean Prosorhochmus americanus (Hoplonemertea)

  • S. A. Caplins
  • C. J. Friedline
  • B. L. Brown
  • J. M. Turbeville
Microsatellite Letters


Thousands of microsatellite loci were identified in the nemertean Prosorhochmus americanus through enrichment-isolation and next-generation sequencing protocols. We tested 48 loci for amplification and subsequently genotyped 17 loci in 30 individuals from three North American populations. Most loci were polymorphic within and across populations; six were monomorphic. The number of alleles per locus ranged from 1 to 9 (mean 4.35, SD 2.37). Cross-species amplification in four nemertean species revealed extended marker utility. These loci will enable investigations of colonization potential and natural history in this selfing, putatively introduced species.


Self-fertilization Nemertean Colonization 



Funding was provided by the Virginia Academy of Science and the VCU Department of Biology. SAC was supported by the NSF GRFP under Grant No. DGE-1147383.

Supplementary material

12686_2014_380_MOESM1_ESM.txt (3 kb)
Supplementary material 1 (TXT 3 kb)


  1. Chybicki IJ, Burczyk J (2009) Simultaneous estimation of null alleles and inbreeding coefficients. J Hered 100:106–113CrossRefPubMedGoogle Scholar
  2. Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. Methods Enzymol 395:202–222CrossRefPubMedGoogle Scholar
  3. Maslakova SA, Norenburg JL (2008) Revision of the smiling worms, genus Prosorhochmus Keferstein, 1862 and description of a new species Prosorhochmus belizeanus sp. nov. (Prosorhochmidae; Hoplonemertea, Nemertea) from Florida and Belize. J Nat Hist 42:1689–1727CrossRefGoogle Scholar
  4. Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. A poor man’s approach to genotyping for research and high-throughput diagnostics. Nat Biotechnol 18:233–234CrossRefPubMedGoogle Scholar
  5. Turbeville JM, Caplins SA (2010) First record for Prosorhochmus americanus (Nemertea Hoplonemertea) in Virginia with notes on its natural history and morphology. Banisteria 35:61–65Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • S. A. Caplins
    • 1
  • C. J. Friedline
    • 2
  • B. L. Brown
    • 2
  • J. M. Turbeville
    • 2
  1. 1.University of California DavisDavisUSA
  2. 2.Virginia Commonwealth UniversityRichmondUSA

Personalised recommendations