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Characterization of 14 microsatellite loci for the pan-oriental narrow-mouthed toad, Microhyla ornata (Amphibia: microhylidae)

Conservation Genetics Resources volume 7, pages 33–35 (2015)Cite this article

Abstract

Fourteen polymorphic microsatellite loci were isolated in the pan-oriental narrow-mouthed toad, Microhyla ornata, which is one of the smallest anuran species. We genotyped one population from Lishui, Zhejiang Province, China (N = 29). The results showed that the mean number of observed alleles per locus was 11 (with a range from 8 to 15). Observed and expected heterozygosity values ranged from 0.250 to 0.931 and from 0.538 to 0.866 respectively. One locus (MO10) showed significant departure from Hardy–Weinberg equilibrium and no linkage disequilibrium was found among any pairwise loci. These highly informative microsatellite markers should be useful for population genetic analyses of M. ornata and other species in Microhylidae.

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Acknowledgments

This work was funded by the Natural Science Foundation of Zhejiang Province (LY13C030004). We thank Chao Zheng for his assistance with field work and Prof. Huabin Zhao (Wuhan University) for constructive comments on the early drafts of this manuscript.

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Authors and Affiliations

  1. College of Ecology, Lishui University, Lishui, 323000, China

    Li Wei, Weiwei Shao & Zhihua Lin

  2. Guangdong Entomological Institute/South China Institute of Endangered Animals, Guangzhou, 510260, China

    Linmiao Li & Jinping Chen

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  1. Li Wei
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  2. Linmiao Li
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  3. Jinping Chen
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  4. Weiwei Shao
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  5. Zhihua Lin
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Correspondence to Zhihua Lin.

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Wei, L., Li, L., Chen, J. et al. Characterization of 14 microsatellite loci for the pan-oriental narrow-mouthed toad, Microhyla ornata (Amphibia: microhylidae). Conservation Genet Resour 7, 33–35 (2015). https://doi.org/10.1007/s12686-014-0334-1

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  • DOI: https://doi.org/10.1007/s12686-014-0334-1

Keywords

  • Microsatellite
  • Microhyla ornata
  • Genetic diversity
  • Polymorphism