Isolation via 454 sequencing and characterisation of microsatellites for Acacia montana (Fabaceae), Mallee wattle: an endemic shrub from southeastern Australia
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We developed eight new polymorphic microsatellite markers for Acacia montana, a bushy shrub from south-eastern Australia used in revegetation projects. Initially, primers were designed for 32 loci identified from 454 shotgun sequencing. Eight loci amplified successfully and were polymorphic when screened on 40 individuals, with observed heterozygosity ranging from 0.250 to 0.789 and the number of alleles per locus from 4 to 13. These loci will be useful in understanding the genetic variation and structure of A. montana in its current fragmented range.
KeywordsAcacia montana Mallee wattle Microsatellites 454 GS-FLX Shotgun sequencing Seed production area
We acknowledge the assistance and support of Alison Fitch from Flinders University and Lan Li from CSIRO Plant Industry for technical assistance. The Goulburn Broken and Murray Catchment Management Authorities for leaf collections. This project was funded by the joint resources of the Centre for Australian National Biodiversity Research and Goulburn Broken Catchment Management Authority.
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