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An evaluation of primers for microsatellite markers in Scarlet Macaw (Ara macao) and their performance in a Peruvian wild population

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Primer pairs were designed for 41 di-nucleotide microsatellite loci identified from across the full genome of the Scarlet Macaw (Ara macao). We present the best 30 polymorphic loci with 5–22 alleles, 3–14 effective alleles and expected heterozygosities of 0.669–0.930. These markers will facilitate population genetic and conservation genetic studies on macaws.

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  • Meglécz E et al (2014) QDD version 3.1: a user friendly computer program for microsatellite selection and primer design revisited: experimental validation of variables determining genotyping success rate. Mol Ecol. doi:10.1111/1755-0998.12271

    Google Scholar 

  • Olah G, Vigo G, Heinsohn R, Brightsmith DJ (2014) Nest site selection and efficacy of artificial nests for breeding success of Scarlet Macaws Ara macao macao in lowland Peru. J Nat Conserv 22:176–185. doi:10.1016/j.jnc.2013.11.003

    Article  Google Scholar 

  • Peakall R, Smouse PE (2012) GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research—an update. Bioinformatics 28:2537–2539. doi:10.1093/bioinformatics/bts460

  • Seabury CM et al (2013) A multi-platform draft de novo genome assembly and comparative analysis for the Scarlet Macaw (Ara macao). PLoS One 8:e62415

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Van Oosterhout C, Hutchinson WF, Wills DP, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538

    Article  Google Scholar 

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This research was supported by funds from the Rufford Small Grant Foundation, Loro Parque Foundation, and The Australian National University. Thanks for technical support to Christine Hayes, Robyn Shaw, and Monica Ruibal. Samples were collected under research permits from the Servicio Nacional de Áreas Naturales Protegidas (SERNANP) in Peru.

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Correspondence to George Olah.

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Olah, G., Heinsohn, R.G., Espinoza, J.R. et al. An evaluation of primers for microsatellite markers in Scarlet Macaw (Ara macao) and their performance in a Peruvian wild population. Conservation Genet Resour 7, 157–159 (2015).

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