Conservation Genetics Resources

, Volume 7, Issue 1, pp 1–4 | Cite as

Characterization of fourteen single nucleotide polymorphism markers in the Manila clam (Ruditapes philippinarum)

  • Hongtao Nie
  • Lianhui Liu
  • Jianfeng Ding
  • Xiwu YanEmail author
Technical Note


Fourteen single nucleotide polymorphisms (SNPs) were developed and characterized from expressed sequence tag sequence of the Manila clam, Ruditapes philippinarum. All loci had two alleles and the minor allele frequency ranged from 0.146 to 0.467. The observed and expected heterozygosities ranged from 0.259 to 0.792 and from 0.252 to 0.503. These are the first SNPs developed for assessing genetic variation and population structure in the Manila clam and will provide a useful complement to currently available genetic markers.


Ruditapes philippinarum Manila clam SNP Tetra-primer ARMS-PCR 



The study was supported by grants from the National Natural Science Foundation of China (31302183), the Modern Agro-industry Technology Research System (CARS-48), and the National High Technology Research and Development Program (863 Program) (2012AA10A410-2).


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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Hongtao Nie
    • 1
    • 2
  • Lianhui Liu
    • 1
  • Jianfeng Ding
    • 1
  • Xiwu Yan
    • 1
    Email author
  1. 1.Engineering and Technology Research Center of Shellfish Breeding in Liaoning ProvinceDalian Ocean UniversityDalianChina
  2. 2.Key Laboratory of Mariculture and Stock Enhancement in North China’s Sea, Ministry of AgricultureDalian Ocean UniversityDalianChina

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