Abstract
The bryozoan genus Watersipora includes rapidly invading species that are becoming common globally. We used paired-end Illumina sequencing to identify thousands of potentially amplifiable microsatellite loci, enabling researchers to track patterns of the invasive spread, and to facilitate ecological and evolutionary question setting. We describe variability of nine loci within recently introduced populations of two Watersipora species in California.
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References
Brownstein MJ, Carpten JD, Smith JR (1996) Modulation of non-templated nucleotide addition by Taq DNA polymerase: primer modifications that facilitate genotyping. Biotechniques 20(1004–1006):1008–1010
Castoe TA, Poole AW, de Koning APJ, Jones KL, Tomback DF, Oyler-McCance SJ, Fike JA, Lance SL, Streicher JW, Smith EN, Pollock DD (2012) Rapid microsatellite identification from illumina paired-end genomic sequencing in two birds and a snake. PLoS ONE 7:e30953
Koressaar T, Remm M (2007) Enhancements and modifications of primer design program Primer3. Bioinformatics 23:1289–1291
Mackie JA, Darling JA, Geller JB (2012) Ecology of cryptic invasions: latitudinal segregation among Watersipora (Bryozoa) species. Sci Rep 2:871. doi:10.1038/srep00871
Ryland JS, De Blauwe H, Lord R, Mackie JA (2009) Recent discoveries of alien Watersipora (Bryozoa) in Western Europe, with redescriptions of species. Zootaxa 2093:43–59
Acknowledgments
Leslee Parr (SJSU), Frank Cipriano (San Francisco State University Genomics/Transcriptomics Analysis Core), Robert Macey (Profile Genomics), Dustin DeMeo (Profile Genomics, SJSU), Maria Bangal (SJSU), Cerise Chen (California Academy of Sciences), Reggie Blackwell (HSU), Jonathan Geller (Moss Landing Marine Laboratory), Steve Lonhart (South West Fisheries Science Center, NOAA), Alexander Poole (University of Colorado School of Medicine), Louise McKenzie (University of NSW), and the Data Intensive Academic Grid (DIAG) support staff (University of Maryland Institute for Genome Sciences) provided assistance or resources. Computation was conducted on the National Science Foundation funded MRI-R2 project #DBI-0959894. Though this study has been subjected to US EPA administrative review and approved for publication, its content does not necessarily reflect official Agency policy. Funding: US National Science Foundation (award #1061695), and California State University COAST consortium.
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12686_2014_286_MOESM1_ESM.txt
Potentially amplifiable loci output—watersiporid bryozoan, Watersipora subtorquta (1,000 primer pairs from IPE). Sequencing representation and other parameters describing primer pairs are described in Castoe et al. (2012). This is a tab-delimited text file that can be imported into a spreadsheet (TXT 156 kb)
12686_2014_286_MOESM2_ESM.txt
Potentially amplifiable loci output—watersiporid bryozoan, Watersipora n. sp, (1,000 primer pairs from IPE). This is a tab-delimited text file that can be imported into a spreadsheet (TXT 153 kb)
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Mackie, J.A., Wostenberg, D., Doan, M. et al. High-throughput Illumina sequencing and microsatellite design in Watersipora (Bryozoa), a complex of invasive species. Conservation Genet Resour 6, 1053–1055 (2014). https://doi.org/10.1007/s12686-014-0286-5
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DOI: https://doi.org/10.1007/s12686-014-0286-5